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We narrowed to 33 results for: Dre recombinase

Showing: 21 - 33 of 33 results

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors...vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available as ready-to-use AAV vectors...

  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... (Howerton at al. 2014). Addgene has several recombinase-expressing ready-made AAV vectors which can be...Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress." The EMBO journal.... Bahi, Amine, Vijay Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of ...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...its expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...expression in the central nervous system (CNS). To address this technological gap, a trio of recent papers...isoform-specific expression of Bdnf resulted in hundreds of differentially expressed genes between Bdnf...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...sequence of interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription...off-target effects, a distinct contrast to the hundreds of observed shRNA off-targets. They subsequently...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also ...hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. Find these AAVs...

  7. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...PMCID: PMC4165888. 6.Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic...2012): 404-414. PubMed PMID: 21567983. 10.Leifer, Andrew M., et al. "Optogenetic manipulation of neural ...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing...all-in-one versions which express self-excising Cre recombinase from the same transgene as the Brainbow cassette...signaling. For more information on DREADDs, please see the Roth lab's DREADD users blog. New CRISPR plasmids...alleles off or on enables this method to rapidly address questions of tissue-specificity and cell autonomy...bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs have developed a new...al., ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several...Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids on his own, UNC-Chapel Hill's Bryan Roth...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...specialized system with parts including site-specific recombinases, regulatory elements, developmental regulators... constructs (Martella et al., 2017). If you can dream up an experiment, MoClo can help you achieve it!...://doi.org/10.1101/2024.02.16.580396. Martella, Andrea, et al. “EMMA: An Extensible Mammalian Modular ...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...lentiviral vector, but transient expression of Cre recombinase allows for deletion of the inserted transgenes...mice. Cre-deletable or Tet-inducible lentiviruses address some of these concerns, but overall integrating...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...species. Indeed, in order to use MAGE, the λ Red recombinase enzymes need to be expressed and the native methyl-directed...plasmids (dubbed pORTMAGE) expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator...system needs to be repressed in the host strain. To address this problem, Csaba Pál’s lab has created a set...

  12. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...conditional mutant mouse brain that expressed Cre recombinase only in pyramidal cells of the hippocampal region...viral proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally... an anterograde tracer and injected at several hundred sites across the entire mouse brain. Traced neurons...PubMed Central PMCID: PMC2698947. Merkel, S.F., Andrews, A.M., Lutton, E.M., Mu, D., Hudry, E., Hyman, ...

  13. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...of sequence-specific enzymes (e.g. nucleases, recombinases) which irreversibly excise or invert a specific...for detection of pre-mRNA splicing   The Gideon Dreyfuss lab has developed rapid-response luciferase (firefly...
Showing: 21 - 33 of 33 results