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We narrowed to 47 results for: Drosophila

Showing: 21 - 40 of 47 results

  1. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    .... They can also be introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR...Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...the Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells...Mol Biol. 2014. PubMed PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege ...expression backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter...rather than a mixed sample. TRIBE was developed in Drosophila, and further work is needed to assess its function...plasmids optimized for dCas9-VPR expression in drosophila and yeast. Chavez A, et al. Nat. Methods 2015...

  3. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...increases knock-in efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation...PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https...

  6. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

  7. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Fragmid was developed for mammalian systems and Drosophila (McGee et al., 2023). Fragmid developers noticed...described above works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate...

  8. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Is there any AAV serotype that can be used in Drosophila? A: Not to our knowledge. Disease Applications...

  9. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light is another way...

  11. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and jGCaMP8...

  13. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon,...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...can be used to replace genes in yeast, mice, or Drosophila through homologous recombination. The concept...

  17. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...sources – voltage-gated potassium channels found in Drosophila to phosphatases from sea squirts. This design...

  18. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...genes: deletion mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002...

  20. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...Washington University whose research focuses on Drosophila-parasite pathogen interactions.  ...
Showing: 21 - 40 of 47 results