We narrowed to 47 results for: Drosophila

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

The Doudna lab recently described a new, thermostable Cas9 variant, GeoCas9. Learn how you can use GeoCas9 in your next genome engineering experimetn.

Sept. 14, 2017, 12:40 p.m.

New fluorescence resonance energy transfer (FRET)-based calcium biosensors and FRET-based sensors for the intracellular imaging of transition metal ions.

May 6, 2014, 1:07 p.m.

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

Jan. 18, 2018, 2:34 p.m.

Two techniques developed by Addgene depositors have made it possible to track mRNA translation at the single molecule level using fluorescent proteins.

Aug. 1, 2017, 1:15 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.

Tim Miles, the guest speaker at our recent AAV webinar, and our webinar panel of experts, is finishing up their Q&A session.

May 17, 2022, 1:15 p.m.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

Dec. 6, 2018, 1:34 p.m.

Learn about a variety of AAV encoded calcium biosensors available from Addgene.

Aug. 10, 2023, 1 p.m.

Find the latest plasmids and viral vectors to available at Addgene in the October 2020 hot plasmids blog post!

Oct. 13, 2020, 1:15 p.m.

Learn about the different open resources for C. elegans like OpenWorm, microPublication, and 3'UTRome, and find C. elegans tools at Addgene.

July 18, 2019, 12:55 p.m.

Find out how you can use new CRISPR tagging plasmids from the Allen Institute for Cell Science to tag your cellular structure of interest.

April 6, 2017, 1:02 p.m.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

Aug. 29, 2024, 1:15 p.m.

A review of the types of voltage indicators available for use as well as how viral vectors aid in the implementation of these systems.

Jan. 30, 2024, 2:15 p.m.

Applying for a PhD post-COVID? Our guest blogger walks you through how to use the virtual/hybrid options to your advantage!

Jan. 5, 2023, 2:15 p.m.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Dec. 1, 2016, 3:30 p.m.

Curious about the history of C-myc, one of the most common antibody tags? Learn all about it here!

Jan. 19, 2023, 2:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.