We narrowed to 35 results for: FLP

1. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ...on the Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Interview: Ed Boyden...

2. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether lab, now available...

3. Rabies and Neuronal Tracing

   Type

   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ... to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression...

4. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory ...

5. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   Published

   Feb. 17, 2022, 2 p.m.

   ...approach proved a challenge. The old standby of CRE/flp didn’t work – it turns out these tyrosine recombinases...

6. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ...proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA...ssDNA. Here, we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018)...

7. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ...split GAL4 architecture or combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al.   ...

8. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

   Type

   Blog Post

   Published

   Oct. 17, 2019, 12:28 p.m.

   ...through site-specific recombinases such as Cre or Flp that induce DNA recombination at defined recognition...

9. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   Published

   Jan. 6, 2015, 5:21 p.m.

   ...developed three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre...

10. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction site...particular site with fragment length polymorphism (RFLP) analysis. In this process, if your mutation introduces... duplication will evade detection in the initial RFLP analysis simply because the introduced restriction...

11. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type

    Blog Post

    Published

    Jan. 16, 2024, 6:13 p.m.

    ...gene pXPR_068 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 L-CHIME Knocks out a single...sequentially pXPR_220 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 Table 1: X-CHIME systems Proof...

12. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available...

13. New Viral Vectors - Winter 2025

    Type

    Blog Post

    Published

    Jan. 28, 2025, noon

    ...Optogenetics Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep ...

14. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type

    Blog Post

    Published

    Nov. 27, 2018, 2 p.m.

    ...additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will be used to create gene deletions of important...

15. CRISPR/Cas9 FAQs Answered!

    Type

    Blog Post

    Published

    March 13, 2014, 4:08 p.m.

    ... doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong et. al (2013) Science...