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  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether lab, now available...

  3. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory ...

  5. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...approach proved a challenge. The old standby of CRE/flp didn’t work – it turns out these tyrosine recombinases...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...split GAL4 architecture or combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al.   ...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA...ssDNA. Here, we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018)...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...developed three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre...

  10. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction site...particular site with fragment length polymorphism (RFLP) analysis. In this process, if your mutation introduces... duplication will evade detection in the initial RFLP analysis simply because the introduced restriction...

  11. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...gene pXPR_068 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 L-CHIME Knocks out a single...sequentially pXPR_220 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 Table 1: X-CHIME systems Proof...

  12. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ... doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong, et. al, 2013 Science...

  14. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Optogenetics Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep ...
Showing: 21 - 35 of 35 results