We narrowed to 82 results for: GRI;
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TypeBlog PostPublishedNov. 15, 2017, 3 p.m....full, compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-containing... 22428975. PubMed Central PMCID: PMC3640491. 2. Grimm, D., et al. "Titration of AAV-2 particles via a ...
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New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....used these Opto-RhoGEFs to study cell junction integrity in the vascular barrier of the endothelium, but....org/10.7554/eLife.84364. Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....development of disease-resistant crops to increase agricultural yield (Wang et al., 2016; Wang et al., 2014)..., Gao C (2020) Applications of CRISPR–Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA...Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development and characterization of sindbis virus... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ...Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin...receptor2 Laminin receptor (LR)3 Putative integrin3 1Wu, Miller, et al., 20062Pillay et al.,... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under Creative Commons...https://doi.org/10.1128/JVI.76.22.11605-11611.2002 Griffin, J. M., Fackelmeier, B., Fong, D. M., Mouravlev... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m.... labeling tag, from Roger Tsien’s lab in 1998 (Griffin et al., 1998), this 15 amino acid peptide contains...https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin, B. A., Adams, S. R., & Tsien, R. Y. (1998). Specific... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....have 1) the courage to make mistakes and 2) the integrity to recognize these mistakes quickly. However, ... for itself but we're now in a rift where our integrity is questioned and cuts to life-saving research... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m....heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins. The goal of...Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D (2018) Engineered... -
Addgene Launches Partnership with IPI
TypeBlog PostPublishedJune 13, 2023, 1:15 p.m....recombinant antibodies. The first collection, a set of integrin antibodies, will be available on Addgene’s website... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m...., primarily in West African countries. Even that grim estimate might prove to be an underestimate. Given... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m..... The first IPI collection, 31 rAbs targeting integrins, will be released early this year. In September... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....of back country skiing, fly fishing, and local grizzly bear attacks. He described one such recent attack... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....excellent—some say “cushy”—tenured faculty position for the grind of corporate life”. In fact, there are a lot of... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m.... bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim -... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....your experimental planning, you need more nitty-gritty details — how can I multiplex this enzyme? What... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General Method... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....set you up for success in the detail-driven daily grind of science. References and Resources Images...