...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...TRIBE is the low input required - McMahon et al., report using as few as 150 fly neurons as their starting...delivery of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston...Drosophila cells, targets 13,501 fly genes with at least three independent gRNAs, each chosen strategically to ...

... which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that ...G-protein-coupled receptors (GPCRs)! Aside from these three senses, GPCRs play roles in initiating signaling...transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma (Gγ)....lab also assembled a collection of plasmids to elucidate the role of 14 of the 16 non-visual heterotrimeric...

...buffered against the effects of copy number on reporter gene expression. On the other hand, using a constitutive...number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing genes of interest...stabilized promoters perform during these types of fluctuations. When cells are dividing rapidly, multiple rounds..., the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially. When...

...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...suitable for spatially limited studies and can fluctuate with changing culture conditions. The use of low...far-red/red light-responsive system is spread across three plasmids, with the response regulator encoded in...

...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds...two-photon microscopy to detect action potentials and subthreshold events, including miniature excitatory postsynaptic...deliver ASAP5 to the cortex and imaged voltage fluctuations in awake behaving mice through two-photon microscopy...

...Associate Professor at Boston College. National reports stress the importance of providing authentic research...complementation should only occur when galactose replaces glucose as the carbon source. Student results have shown...College. Students in the course work in teams of three in sections of 15 students. Within each section,...section, teams are assigned different groups of three genes selected from different parts of the Met pathway...

...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...Chris Humphrey from Jobs on Toast is based in the United Kingdom and scientists there would be lucky to ...

..., as well as the Editorial Board of Scientific Reports and PLoS One. Additionally, you are involved in...as transcription and epigenetic regulation. To elucidate molecular mechanisms of regulation of genome functions...that in the US for me. In addition, I've been very lucky to work with excellent colleagues in my lab. On ...tendency is one of the reasons why we have only three biology-related Nobel prizes, out of which only ...

...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and transfer ...Fischer A, Cornetta K, Galacteros F, Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. ...

...concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and... just described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin...Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced...

...colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...individual cells. The Brainbow-1.0 construct contains three fluorescent proteins: RFP (red), YFP (yellow) and...proteins, with recombination events specified by three loxP variants, but the principles remain the same...absent; stochastic Cre excision results in one of three recombinations, and subsequent recombinations cannot...recombination events leads to a rainbow of colors. With three copies of the construct, one would expect ten colors...) From the seven resulting proteins, they chose three with low levels of both fluorescence and sequence...Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835...

..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...Easi-CRISPR works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...Quadros et al. saw success with Easi-CRISPR across three independent core facilities for 13 projects, with...

...β-like-cells [37, 38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation...from fibroblasts, human keratinocytes from hair pluck, peripheral blood cells, and renal epithelial cells... damage results in the transdifferentiation of glucagon-producing α-cells into insulin-producing β-like-cells...Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration. Nat Rev Mol Cell Biol, 2011...

...), and ability to metastasize. There have been reports of clinical trials going amiss and patient deaths...progression. Dunn et al in 2002, introduced the “Three Es of Immunoediting” nomenclature. 1. Eliminate ...385.9967 (2015): 517-528. PubMed PMID: 25319501. 7. Palucka, Karolina, and Jacques Banchereau. "Cancer immunotherapy...