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We narrowed to 814 results for: LAS

Showing: 21 - 40 of 814 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection targets...precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song... proteins for mammalian cell expression. These plasmids will be useful not only for future studies of ...proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid, ...al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions that...potential, ASAP5 is a great new option. Find ASAP5 plasmids here! Hao, Y. A., Lee, S., Roth, R. H., Natale...

  2. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here! References...antibiotic resistance markers (indicated here by plasmid color), allowing you to easily select for the expression...desired, recombined construct. Read our recent Plasmids 101 Post on CcdB for more information. How to ...Method A: recombination of an attB-PCR product or plasmid with an attP donor vector. In this case, we would... to use the Snapgene program to design Gateway plasmids.    Figure 3: Method A to create an entry...entry clones can be used with a large variety of plasmids. Speed The Gateway system enables the generation.... Take a look at some of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ... you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over...over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...modifications Learn more molecular biology in our Plasmids 101 eBook! Researchers use various techniques ...for your protein of interest is inserted into a plasmid expression vector that is then transformed into...being able to help the scientific community share plasmids.   Additional Resources Thermofisher Protein ...Addgene.org Bacterial expression systems Search for plasmids by expression system ...

  5. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...use light to regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI...Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org: Inducible...Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...over 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert Campbell’s lab...editing for precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design of ...minimal background.   Find these C. elegans mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar ...disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah...Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium...

  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome...fused to a DNA methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021)....expression off. Plus, DNA methylation is a long-lasting repressive marker, allowing the team to develop...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...factors impacting prime editing and found the protein La (a small RNA-binding exonuclease protection factor...They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7 shows enhanced...the improved efficiency is at least in part due to La binding and stabilizing the 3’ end of the pegRNA,...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime...

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101 eBook Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human Cells ...In the last decade, the use of degron tags has become increasingly popular for the modulation of endogenously...tag and why should I use one?   Degron tags are a class of protein tag which can be added to either the ...cloned in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of...

  11. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...NEB’s DAD Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ...

  12. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...conjugative mobilization of broad host-range IncQ plasmids." Plasmid 62.2 (2009): 57-70. PubMed PMID: 19465049...page Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...family-RP4 conjugation system. This system can deliver plasmids to both Gram-positive and Gram-negative cells.... Wang lab further developed a modular suite of plasmids (pGT) with various replicative origins that can...genera across four phyla that have taken up the plasmid through conjugation in vivo studies. However, they... tools for microbiome studies.  Find the MAGIC plasmids here Paper-based synthetic biology approach to...conjugation. The labs screened for the host-range of the plasmids and suitability of various antibiotic resistance...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...consider your plasmid's compatibility with one or more other plasmids. Generally speaking, plasmids with the...enabling a plasmid to reproduce itself as it must to survive within cells. The replicons of plasmids are generally...cell. Figure 1: A plasmid map showing the standard features of a plasmid. So, how do you choose...have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene...avoid plasmid incompatibility In other words, the best choice of ori depends on how many plasmid copies...a rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an ...purify your plasmid DNA: The insert Bacteria tend to maintain fewer copies of plasmids if they contain...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts. This...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...head over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively...offers advantages for cellular delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... cells were transfected with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...

  16. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...post: Improved voltage indicator: JEDI-2P Novel class of light-gated potassium channelrhodopsins Myc-tag... you can also find several other constructs in plasmid form. Fig. 1: Summary of the approach and...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue.../doi.org/10.1016/j.cell.2022.07.013.   Novel class of light-gated potassium channelrhodopsins by: Mike...Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins, named...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here. Here's what you'...easily be applied to other systems.    Find BLITZ Plasmids here! Xiong, et al. eLife. 2021. https://doi.org...from Goertsen D, et al., 2022.  Find M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience....have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and microbial...Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol. 2022. https:/...

  18. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...ability to knock out multiple genes in parallel. Lastly, this Cas12a-based screening method was used to...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative... ladders. Image from Santilli, et al. 2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ... Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% knockdown efficiency across ... genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori...Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...Addgene blog:  Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR...process known as plasmid multimerization. Plasmid Multimerization Early studies on plasmid topology recorded...ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric form of the plasmid. Have... monomer of Plasmid 12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The plasmid can exist on... have been utilizing full plasmid sequencing services to verify the plasmids, including long-read sequencing... the sequencing results for their plasmids, they found the plasmids were much longer than expected, and...tandem. Such plasmids are known as multimers, and they are the result of multiple plasmid copies combining...
Showing: 21 - 40 of 814 results