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We narrowed to 198 results for: LRE

Showing: 21 - 40 of 198 results

  2. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...resources discussed in post one of this series already provided or check the materials and methods of ...empirically but it’s best to try those that have already been published first. Because behavioral optogenetics...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...), that comes in contact with the cells should already be at 37 °C. Most labs have a water bath constantly...master stock instead of ‘borrowing’ cells that have already been passaged many times and could be contaminated.Learn...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...be demonstrated (or invented), its behavior has already been successfully reproduced by several other labs... And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools...

  5. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...are all very valid questions, and often make an already complicated process even more difficult. I’ve learned...didn’t have to worry about traveling during an already stressful time, and I was more relaxed during both...

  6. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...are only applicable to those positions you have already held. Imagine this situation: You’ve spent hours...this new opportunity much different? Might you already have leadership skills that are just not being ...

  7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...access to intracellular targets. If your target is already on the extracellular side of the membrane, then...to start with conditions and reagents that have already been used successfully. If there isn’t information...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... research so you can go to where your audience already is.  You’ll also want to keep in mind that access... make it difficult to reach people who are not already actively interested in scicomm. Difficult to do...

  9. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... open source assembly algorithms meant that we already had a clear idea of the necessary steps to perform...instrument (hundreds of plasmids each week!), we already planned to use cloud computing resources. Now, ...

  10. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...timesaver for them if you come to a training having already read a paper or protocol. If you have questions...ask questions and/or receive help until you’ve already started a time sensitive experiment. Ready to ...

  11. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...amounts of digital data during their work. It is already becoming impossible to keep track of it all in ... smooth as all data is organized within an ELN already. Investing some time in deploying a software solution...

  12. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ... results (14). Some loss of trust, I fear, has already come to pass. It may only be by advocating for ... more involved in their communities (something already encouraged by “Broader Impacts” requirements for...

  13. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...selection of fluorophores. If the available colors are already reserved for other markers in your panel, and thus...after viability staining and surface labeling are already done. Intracellular labeling can be subdivided ...

  14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...regulate a desired phenotype. In fact, CRISPR has already been used to engineer the genome of many plant ... yellow net rhabdovirus (Ma et al., 2020) have already been shown to be efficient ways to deliver CRISPR-Cas9...

  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...protein somebody made because they forgot it was already in the freezer… you get the idea. What if there...expression with the same imaging workflows you already use. Or you can really capitalize on the dye’s ...

  16. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...oh-so coveted asterisk of significance? You’ve already done the heavy lifting, so don’t let the statistical...design your experiment, instead of after you’ve already run it.) You don’t need to be a mathematician to...

  17. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...eOPTI-CGBE and cOPTI-CGBE Many optimizations had already been proposed for other base editors, so the Yidi... increased even in chromatin regions that were already accessible. It's not entirely clear why this is...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... need a gradient gel, the decision is probably already made: gradients are extremely difficult to handcast... less waste. However, they also add time to an already lengthy protocol, require the use of hazardous ...

  19. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...111 countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...
Showing: 21 - 40 of 198 results