We narrowed to 217 results for: LUM

...to as many lab alumni as you can or schedule video/calls if they are not local. Alumni meetings can be... too long or do they leave prematurely? Do lab alumni stay in the same field taking projects with them...remaining close colleagues of the PI? Or do lab alumni leave the field or get pushed out by the PI? What...One scientist who followed my advice to interview alumni of the lab was told that the PI removed a woman... happen to you. As you speak to lab members and alumni, don’t ever disregard warning signs of unethical...supporting career development and growth. Have lab alumni gone into both academic and non-academic careers...

...kits and lesson plans adapted from her own MIT curriculum. Drawing on current questions being asked in ...field of STEM education worldwide. The BioBuilder curriculum is now taught in almost every state in the U....BioBuilder experience With its intentionally malleable curriculum, BioBuilder takes cutting-edge research projects... as a final project. BioBuilder’s open-access curriculum begins with students reading comic strips and...their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes by emphasizing... by O’Reilly Media. BioBuilder's open-access curriculum available on Amazon Inspire.    Many thanks...

...YFP and use an affinity column for purification. After binding YFP to the column, you wash several times... use OptoNBs. You fill a column with OptoNB coated beads and wrap the column with blue LED lights. When...their target protein is altered by blue light illumination. This ability to reversibly bind and release...pathway. What’s next for OptoNBs? While OptoNBs illuminate a path to light-controlled protein binding, they...

...bind the IgG heavy chain. Running the serum over a column of resin crosslinked to protein A /G will bind ... antigen. In this case, the serum is run over a column containing the target protein, and any antibody...recognizing the target protein remains bound to the column while the rest of the proteins are eluted. Then...Then, the antibodies are dissociated from the column and from the target protein. Pre-adsorption Finally... pre-adsorb the antibodies, pass them through a column containing immobilized serum protein from other...-react with the serum proteins pass through the column, resulting in a polyclonal antibody population ...

... genome copies/mL (GC/mL)), and total injection volume. You’ll often hear that “high titer” is required... It’s the optimal number of viral particles per volume required to express the gene of interest, in the...expression system for both the titer and total injection volume.  There are a few factors to consider. First, ..., the lower the titer and vice versa. The total volume needed, however, increases with the size of the...- and as your titer changes, so may your needed volume (de Backer et al., 2010).  Second, high efficiency...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic...

...can be measured using a technique known as Bioluminescence Resonance Energy Transfer (BRET). BRET is similar...BRET is that the energy transfer is between a luminescent donor and a fluorescent acceptor. TRUPATH uses...measures heterotrimeric G protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With... constructs. Cells are then treated with the luminescent enzyme substrate coelenterazine, followed by ...endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2 will be...

...plasmid per row, as outlined by the column headers. Required columns are shown in red in the spreadsheet...species can be indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid... If other, indicate type in “Vector Type Other” column. For example, you may enter “donor template vector...the deposit spreadsheet showing the first eight columns and a pop-up instruction box for the “Purpose” ...

...compensate for this, researchers can plate larger volumes of the outgrowth containing the transformants onto...the lacI/lacZ cassette, and illustrates how the volume of the 1 ml outgrowth spread on each transformation...fragments assembled Golden Gate Assembly protocol* (Volume of 1mL Outgrowth plated) Correct assemblies per...Efficiency of assemblies per plate using outgrowth volumes described in Figure 3, with calculated yields from...ºC incubations or cycling.  *Assembly reaction volumes were 20 μl (1, 12 fragments) or 25 μl (24 fragments...

...fluorescent proteins, and luminescent proteins. While fluorescent proteins and luminescence have been widely used... fluorescence or luminescence, which require UV lamps, fluorometers, or luminometers, chromoprotein detection...striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful corals, to name...

...bacteria. Performing your Assemblies Calculate your volumes As you progress through the MoClo assembly levels...efficiency, we recommend you calculate the right volume of each plasmid according to their concentration...    Figure 3: Calculation for the plasmid volume for MoClo experiments. The term ‘insert’ refers...more accurate, but they also work with very small volumes of reagents which lowers experimental costs. If...efficiency. To combat this, you can increase the volume of competent cells you plate after each transformation...

...determine the optimal/minimal injection titer and volume needed to achieve adequate expression in your cells...cells of interest.  Determine injection titers and volumes based on the serotype Since each serotype also ...advisable to try a few different injection titers and volumes to determine the optimal injection parameters needed...will already have a starting “optimal” injection volume and titer to begin with. Now you will mainly only... vector, a range of dilutions and/or injection volumes may need to be tested with each new vector or lot...

... dyes are now commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific...Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc...Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly...permeabilized cells  Cy5NTAc 43 34-62 YFP   Lumio Red Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly...

...vivo.  Read on to learn more about this killer illumination technique! What is a photosensitizer? First...within 30 minutes of exposure. 90 seconds of illumination were sufficient for 100% effective killing of...the dye. Embryos exposed to just 12 minutes of illumination had fewer mCer3-labeled cells in their hearts...photosensitizer protein that generates ROS when illuminated with green light. They found that KillerRed required...

...however, affected by bubbles in wells, variations in volume, or machine surge errors and allow for well-to-...revisited. Biomolecular Detection and Quantification. Volume 14. 2017, Pages 19-28, ISSN 2214-7535, https://...products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, https...Reproducible Data the First Time, Trends in Biotechnology, Volume 37, Issue 7, 2019, Pages 761-774, SSN 0167-7799... to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages 3752–3761, ...

...the volume typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture volume into...resuspension of the collected particles in a smaller volume. Always remove packaging cell debris before proceeding...without ultracentrifugation, you can reduce the volume of cell culture medium on the packaging cells immediately...

...are both great options for this, depending on the volume of culture you chose to work with. You will get... construct at a time. For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium strains...messy, so you want to make sure you have sufficient volume to dip all your plants. Make sure your Agrobacterium... viable. The time this will take depends on the volume of culture you are using and whether you are inoculating...

...Nickel Column: Lyse the bacteria expressing your nanobody and run the lysate over a nickel column. Nanobodies...Nanobodies will bind to the column due to their N-terminal His14-bdNEDD8 tag. After several washes, this...protease, thus releasing purified nanobodies from the column. bdNEDP1 protease can be generated using plasmid...

...chromatography is a popular choice for this. Chromatography columns containing appropriate immobilized ligands are ...buffer exchange can be performed using a desalting column or an ultrafiltration concentrator. As the name... prep to the desired concentration using these columns. If you are conjugating your antibody in a downstream...Current Opinion in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449, ISSN 1359-0294...

...the go-to method is to use spectrophotometry. Microvolume spectrophotometers make it quick and easy to ...Quick note before jumping in — in the world of microvolume spectrophotometers, the Thermo Scientific NanoDrop...References and Resources References Ahlfen SV, Schlumpberger M. Effects of low A260/A230 ratios in RNA preparations...Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers. NEB Tech Note (2019).  Loughrey...

...solution to avoid autofluorescence is offered by bioluminescent proteins. These probes generate light by a ...there is no need to excite the sample. Although bioluminescent probes are usually dim relative to fluorescent...palette of Nano-lanterns for real-time multicolor luminescence imaging." Proceedings of the National Academy...PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of deep tissue in freely moving animals...