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We narrowed to 134 results for: Lta;

Showing: 21 - 40 of 134 results

  1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... multiple sgRNA constructs to be introduced simultaneously in a single plasmid. The functionality of the...of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex control via scRNA...Multiple scaffold RNAs can be designed to allow simultaneous, multidirectional and tunable control of gene...

  3. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...experiences, she realized her niche as a grant consultant to faculty writing research grants, especially...full-time job or want to be an independent grant consultant, building your area of expertise will help you...to mentor and coach you. There are many grant consultants who offer grant training, mentoring and coaching...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...downstream of a functional promoter (gene traps). The resultant gene targeting system could be used to routinely...at once. Therefore, applications that require simultaneous modification of all alleles of a gene, or which...which require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...approach relies on the incorporation of light-oxygen-voltage (LOV) sensing domains from algae into chimeric ...integrating DNA sequences either sequentially or simultaneously. This method, called by the authors the “Clonetegration...repeat-variable di-residue (RVD) module plasmids and simultaneously increases the success rate of module assembly...in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems in different tissues ...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Essentially, any method that results in the bulk of resultant fragments falling between 1 and 3 kb results in...modifications to DNA bases.  Ligate and transform your resultant plasmid libraries into chemically competent E....DNA directly from the colonies. Transform the resultant plasmid DNA into E. coli BL21 (DE3) cells (these...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for both transient expression and ...small insertions. Multiplexing of two targets simultaneously is possible with efficiency >70%. CRMAGE is... delivered on a different plasmid and allows simultaneous multiplex editing of six different genes. This...

  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... can be used to generate multiple mutations simultaneously (Jiang et al. 2013). Genome editing tools for...small insertions. Multiplexing of two targets simultaneously is possible with efficiency >70%. CRMAGE is...knockdown expression of multiple target transcripts simultaneously. Marraffini lab's CRISPRi/a plasmids In addition...

  9. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...at lower temps. This approach only works for facultative thermophiles (high OR medium heat loving), but...clears the way for CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to...

  10. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...multifunctional fibers, embedded, and validated simultaneously in the brain and the intestines of mice. The... To operate both types of implanted fibers simultaneously, a mini-Bluetooth system named NeuroStack was...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...a white color. While CRISPRainbow allows for simultaneous visualization of sites on different chromosomes... color detection In order to label two loci simultaneously, Ma and Pederson created another sgRNA containing...

  12. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    .... phaffii Growth properties Facultative anaerobe Facultative anaerobe Obligate aerobe Number...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires...of fluorescent proteins, imaging four colors simultaneously should be pretty straightforward. Thank You...

  14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic alterations... and animal imaging studies. The ability to simultaneously alter multiple genes via infection with a single...

  15. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based... permit the use of multiple FRET biosensors simultaneously would further increase the amount of correlated...

  16. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...CEPIA (additional versions are available to simultaneously measure calcium changes in the ER, cytosol ...fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination with violet...

  17. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...

  18. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ..., unlike voltages, you can adjust compensation values later in your analysis, unlike voltages.  Figure...

  19. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...perform its helicase and nuclease functions simultaneously, by continuously unraveling dsDNA, pulling ...reeling of ssDNA and unwinding of dsDNA occur simultaneously, which creates a loop in the target strand ...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...cytosine (C) into a uracil (U) base. Later the resultant U-G pair is either repaired by cellular mismatch...enables us to target more than one genomic site simultaneously.     These binary vectors could be used to ...
Showing: 21 - 40 of 134 results