... multiple sgRNA constructs to be introduced simultaneously in a single plasmid. The functionality of the...of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex control via scRNA...Multiple scaffold RNAs can be designed to allow simultaneous, multidirectional and tunable control of gene...

... abundance of thousands of protein variants simultaneously  With increasing sequencing power, scientists...the effects of sequence variants on abundance simultaneously called VAMP-seq, for Variant Abundance by Massively... to a second population. Last, Bernach will simultaneously monitor cell cycle and growth rate using TIMER...

...experiences, she realized her niche as a grant consultant to faculty writing research grants, especially...full-time job or want to be an independent grant consultant, building your area of expertise will help you...to mentor and coach you. There are many grant consultants who offer grant training, mentoring and coaching...

...downstream of a functional promoter (gene traps). The resultant gene targeting system could be used to routinely...at once. Therefore, applications that require simultaneous modification of all alleles of a gene, or which...which require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based...

...Essentially, any method that results in the bulk of resultant fragments falling between 1 and 3 kb results in...modifications to DNA bases.  Ligate and transform your resultant plasmid libraries into chemically competent E....DNA directly from the colonies. Transform the resultant plasmid DNA into E. coli BL21 (DE3) cells (these...

...approach relies on the incorporation of light-oxygen-voltage (LOV) sensing domains from algae into chimeric ...integrating DNA sequences either sequentially or simultaneously. This method, called by the authors the “Clonetegration...repeat-variable di-residue (RVD) module plasmids and simultaneously increases the success rate of module assembly...in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems in different tissues ...

...assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for both transient expression and ...small insertions. Multiplexing of two targets simultaneously is possible with efficiency >70%. CRMAGE is... delivered on a different plasmid and allows simultaneous multiplex editing of six different genes. This...

... can be used to generate multiple mutations simultaneously (Jiang et al. 2013). Genome editing tools for...small insertions. Multiplexing of two targets simultaneously is possible with efficiency >70%. CRMAGE is...knockdown expression of multiple target transcripts simultaneously. Marraffini lab's CRISPRi/a plasmids In addition...

...at lower temps. This approach only works for facultative thermophiles (high OR medium heat loving), but...clears the way for CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to...

...multifunctional fibers, embedded, and validated simultaneously in the brain and the intestines of mice. The... To operate both types of implanted fibers simultaneously, a mini-Bluetooth system named NeuroStack was...

.... phaffii Growth properties Facultative anaerobe Facultative anaerobe Obligate aerobe Number...

...a white color. While CRISPRainbow allows for simultaneous visualization of sites on different chromosomes... color detection In order to label two loci simultaneously, Ma and Pederson created another sgRNA containing...

...follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires...of fluorescent proteins, imaging four colors simultaneously should be pretty straightforward. Thank You...

... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based... permit the use of multiple FRET biosensors simultaneously would further increase the amount of correlated...

...CEPIA (additional versions are available to simultaneously measure calcium changes in the ER, cytosol ...fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination with violet...

...Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic alterations... and animal imaging studies. The ability to simultaneously alter multiple genes via infection with a single...

...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...

..., unlike voltages, you can adjust compensation values later in your analysis, unlike voltages.  Figure...

...perform its helicase and nuclease functions simultaneously, by continuously unraveling dsDNA, pulling ...reeling of ssDNA and unwinding of dsDNA occur simultaneously, which creates a loop in the target strand ...

...cytosine (C) into a uracil (U) base. Later the resultant U-G pair is either repaired by cellular mismatch...enables us to target more than one genomic site simultaneously.     These binary vectors could be used to ...