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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 109 results for: MAX

Showing: 21 - 40 of 109 results

  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry has developed a ...Multisite Gateway compatible destination vector for a maximum of 24 elements assembled into a final transgenesis...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular Medicine has recently...independent gRNAs, each chosen strategically to maximize the likelihood of creating a functional knock-...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...indel frequency. Koblan et al., 2018 BE4max, ArcBe4max, ABEmax   Improved nuclear localization and expression...localization signals and codon usage BE4 to create BE4max and AncBE4max with a 4.2-6-fold improvement in editing...adenine base editing When the Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine...., 2020 SpRY Cas9, available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site... more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly more effective at editing...localization and expression. This base editor was named ABEmax. In 2020, two papers were published describing ...programmable adenine and cytosine editor) by fusing miniABEmax-V82G and Target-AID to Cas9 (Grünewald et al....

  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... continued to improve the maximum efficiency of prime editing. While maximum efficiency isn’t everything... PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized prime editing variants...end of PEmax to produce PE7. PE7 significantly improves editing efficiencies compared to PEmax with normal... originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor sequence to improve expression...be combined with any of the PE2-PE5 strategies. PEmax contains a reverse transcriptase with a sequence... although both enzymes still approach or exceed PEmax editing efficiencies for short, simple edits. PE6c... Innovation Purpose PE2 PE3 PE4 PE5 PEmax PE6 PE7 Pentamutant reverse transcriptase...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...spectrum shows two maxima, one local maximum at just under 500 nm and a global maximum at around 560 nm,... global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If...and emission spectra. Both spectra possess clear maxima where excitation and emission are the most efficient...fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use the tool to display excitation...wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation spectrum (dotted line, Figure...excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where the excitation...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...–5 mL 5–50 µg Midiprep 25–100 mL 20–200 µg Maxiprep 100–500 mL 100–750 µg Megaprep 0.5–2.5 L 1.5... Macherey-Nagel Beads, column, plate Mini, midi, maxi, mega, giga $ Yes —beads, columns, plates, buffers... BAC Millipore Sigma Column, plate Mini, midi, maxi, mega, giga $$ No 96-well plates Some Endotoxin-... Omega bio-tek Beads, column, plate Mini, midi, maxi $ Yes — beads, columns, plates, buffers 96-well ...transfection-grade Promega Beads, column, plate Mini, midi, maxi $$ Some — buffers 96-well plates, automation-friendly...Transfection-grade Qiagen Column, plate Mini, midi, maxi, mega, giga $$ Yes — columns, plates, buffers 96... Thermo Fisher Beads, column, plate Mini, midi, maxi, mega, giga $ Some — buffers 96-well plates, automation-friendly...

  9. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...molecules have a specific wavelength that they maximally absorb. The spectrophotometer measures these absorbances...contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins on the other hand absorb best...nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280 ratio is used ...

  10. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...uniquely positioned to help research institutions maximize their increasingly limited resources: Preserving...resources become even more valuable as labs look to maximize productivity and efficiency with fewer resources...

  11. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...we are processing 159 more requests." Pool and maximize resources nationally and internationally COVID...McGill University. “There is a need to pool and maximize resources nationally and internationally such ...

  12. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...deamination. They generated these editors using the BE4max architecture. The new editors reported in this ...average on-target DNA-editing levels relative to BE4max.  Read the paper in Nature Biotechnology Find ...

  13. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...Kcleave (min-1). SpCas9 has maximum activity from 35-45 °C, while GeoCas9 has maximum activity from 50-70 °C...

  14. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...amino acid sequence of the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and...disease.  Read the paper in Cell Find PE4, PE5, and PEmax plasmids Precise genomic deletions In PRIME-del...

  15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...advancements in gRNA design necessary to achieve maximal on-target and minimal off-target activities have...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  16. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA (2017) A Broad-Spectrum Inhibitor...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016) Naturally Occurring Off-...

  17. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...through several rounds of stripping and reprobing, maximize your signal by starting with the least-abundant...Immediately after transfer, dry the membrane to maximize protein retention. Following this step, most PVDF...

  19. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...1: Left: recombined prime editing tools PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...growth time, temperature, and shaking speed to maximize cell density and plasmid yield as these factors...need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time should be...
Showing: 21 - 40 of 109 results