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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 21 - 40 of 628 results

  1. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...many roles that actively require these skills, including: Science Reporter Scientific Sales Epidemiologist...Careers where you’ll actively use these skills include: Science Reporter Scientific Illustrator Research...outdoor skills are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant...etc) are highly desirable in a range of roles, including: Science Reporter Technical Writer Statistician...position. Questions you’ll want to find answers to include: Will you be sitting at a desk all day or be on...to the content in your Resume and Cover Letter. Include any relevant job specific ‘buzz words’ to ensure...

  2. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be detected...greater than our previous mapping software and now includes Type IIS restriction enzymes that are commonly...tooltip information and sequence analysis tabs including: Maps This is the first tab you’ll land on...enzymes that cut the given sequence. The table includes enzyme names, the locations where the enzymes ...features detected in the plasmid sequence. The table includes feature names, locations, sizes, colors used to...detected in a given nucleotide sequence. The table includes primer names, sequences, binding site locations...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...things you need to include in your manuscript. What information do I need to include in my paper? More ...and your results more valuable. Some journals, including F1000Research, also ask for the raw data behind...good quality and whether they agree with the conclusions of the article. These initiatives are another... to your submission, briefly summarizing the conclusions of your paper. This will help the editor handling... have ignored their comments, they will not be inclined to approve your paper. Rush your submission or...

  4. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...strategies beyond SpCas9, or species not currently included in Green Listed.  Provide the list of genes ...Input” box. Choose from different “Options”, including adding adapter sequences for cloning purposes.... output folder containing several files. These include: Output: A text file containing full information...information about the suggested gRNAs to use, including all information provided in the original reference library...Output_Compact: A text file that describes which genes were included, and how many gRNAs were identified for each gene... gRNA, as well as the suggested gRNA sequence including adapters. Output_UserInputParams: Contains all...from the software, and my hope is to continue including other libraries. As of now I have focused on human...

  5. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...from the company LinkedIn page. This can include news, inclusion, culture and connections. Start your interview... is within a few days after you’ve met. Always include a note about where you met IRL (in real life) and... a connection request to any of these people, including and especially the hiring manager. This is considered.... Follow all the rules of good resume writing. Include examples of concrete accomplishments in both the...the technical and “plus skills” areas. Include a professional picture. It doesn’t have to be a headshot...

  6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...results that ultimately builds up to an overall conclusion(s). In some articles, the data are presented ...to build a case for a certain model or overall conclusion. In others, the experiments are geared towards...possibilities until the results focus in on a particular conclusion. Many papers aren’t always written this way and...the results to see if it leads you to the same conclusion. Personally, I like papers that can end with ...are appreciated) to visually sum up all of the conclusions. In any case, the reader should give a critical... the relation to how they are supporting the conclusions. Look at the figures and write down your own ...

  7. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...experimental processes, results, and subsequent conclusions. Being vigilant about these errors is an important...easy to share. However, the cloning process — including the propagation of plasmids in bacteria — has ...and money on optimizing experiments, to false conclusions.  Figure 1. Potential points where DNA..., indicated by exclamation marks. Error points include places where DNA is being created, such as PCR ...believe may affect the function of the plasmid. This includes point mutations, frame shifts, missing elements...require additional attention during analysis. These include topics such as: locations in a plasmid that are...

  8. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic or .... We do this through a variety of activities, including creating educational materials and lesson plans...to hamper efforts to engage these communities, including those by researchers hoping to increase community...leadership and communication experience. These included taking extra coursework and attending seminars...of organizations in public engagement efforts, including giving public lectures, writing science articles...our experiences in a variety of other settings, including “conventional” academic venues such as scientific...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ..., and include information on tested antibodies against the target. Helpfully, this also includes clone...antibody for flow cytometry experiments. This will include a discussion of the Human Leucocyte Differentiation...approach Genetic knockout-based validation can conclusively prove the ability of an antibody to detect the...separation and reduce any background staining. This can include using: different blocking reagents; titration of...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown is...molecules expressed on human blood leukocytes, including antibodies against these markers. The 371 CD markers...

  10. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...recently, online journal clubs have become popular, including those based on Twitter. At my former academic ...recurrently carved out. This notice typically should include the paper, any supplemental data, software repositories...feedback could be solicited via multiple avenues including casual request via email, in person, or instant...group and format but should almost universally include the questions “Would you attend this journal club...you can point to. Does the feedback you receive include the sense that people learned something new? Did...continually evaluated and assessed for relevance.  Concluding thoughts Journal clubs are a great way for scientists...

  11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ... control for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and...off-target cutting of only a handful of guides. It’s unclear if off-target cuts confound the results of high-throughput...without a reduction of on-target activity, but it’s unclear if this holds true in genome-wide screens.   ...previously not associated with ricin regulation, including almost all genes involved in the production of...non-targeting guides and 6,750 safe-targeting guides were included in the library. Safe-targeting guides were designed...activity, Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting...

  12. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... FP fusions including erroneous quantitation of fusion protein levels. It remains unclear whether the ...chemically distinct subcellular environments, including the organelles that compose the secretory pathway... significant post-translational modifications including glycosylation, disulfide bond formation, and proteolytic...imaging in a variety of cellular compartments including the secretory pathway (Figure 2), the inner membrane... Anatomy and Structural Biology. His interests include the quality control of secretory proteins in the...

  13. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...experiment lacking the proper controls are invariably inconclusive and unreliable. Proper controls provide the ...human cells. From this result one could simply conclude that the shRNA didn’t work, as the expression ...control plasmids Part of planning your experiment includes determining what factors need to be controlled...microscopy images resulting from our experiment that includes the aforementioned internal control and is consistent...to note that optimal experimental conditions, including how much plasmid DNA to use for any individual...plasmid-based experiment, untreated cells should be included as these provide the baseline/standard against...

  14. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... open access publisher has required authors to include a note as to where readers may locate data supporting...information) for assessment of veracity of the conclusions drawn. The main difference with the PLOS policy...the time of manuscript submission. The rationale includes increasing the ability of reviewers (and, once...information is made available to everyone at once, including the generators. This practice doesn’t appear to...The fact that so few authors now query what to include suggests pragmatic adoption (although it might ...access to data is important, what data should be included? We should recognize that storing data in a sharable...

  15. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Project conducted a large scale sequencing and concluded that nearly all disease-linked human genes have...as limbs, the spinal cord, and internal organs including the heart, brain, and lungs (McCusker and Gardiner...constructed several vectors for use in the axolotl including plasmids for use in CRISPR and Tol2 transposase...Some of the most popular tools for studying frogs include egg transplants and microinjections. Injections...fluorescent fusions in a multitude of organisms including Xenopus. Overall, the ability for scientists to...function. Some key genetics research with Arabidopsis include understanding of allelic variation between different...

  16. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...to record keeping. Top priorities in most cases include IP protection, publications and funding. If any...which focused on the adoption of ELNs in labs and included hundreds of researchers. The full story is available...was a significant barrier to ELN adoption. This includes financial outlay, staff hours, troubleshooting...emails. Important collaborative functionalities include comments, notifications, sharing files, assigning... clients, you might need share data with them including reports and protocols. To facilitate this, some...meetings with the support teams behind them.  Conclusion Researchers spend 40% of their time on tedious...

  17. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...single-fluorophore-conjugated antibodies. These methods can include labeling markers with multiple proteins/antibodies...fluorescence-activated cell sorting (FACS) enables you to include further (non-lineage) markers alongside, e.g., ...as proteins of the secretory pathway. Those can include cytokines and chemokines; enzymes like kinases,...solution can be the same). Examples of such targets include transcription factors, histones, and DNA repair...still be easily gated on during the analysis. Conclusion Congratulations! You made it through this blog...Sharpe at Harvard Medical School. His projects include investigating negative regulators of T cells and...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...Symptoms of AIDS include rapid weight loss and an increased risk of infection, including both common infections...at increased risk for other chronic diseases, including dementia, gut disorders, nerve damage, and cardiac...1 is limited to a subset of T-cells, but it is unclear if enough T-cells could be reached to ablate a ...could be used to combat this problem, just as ART includes multiple drugs to lower the odds of developing...near the Cas9 cut site, leading Wang et al. to conclude that some Cas9-derived indels may not ablate viral...

  19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...create knockout alleles in a variety of species, including the standard laboratory mouse. It’s also made ...bacteria. But CRISPRing typical mouse models, including creating Cre-dependent conditional alleles, has...gRNAs. A variety of other undesired outcomes, including one loxP site insertion and one NHEJ-repaired ...problem. Briefly, you design your donor template to include a T7 promoter upstream of the left homology arm...your ssDNA. Note that the T7 promoter will not be included in the final ssDNA template. Figure 2: ...single-stranded DNA production services or kits, including the IDT Megamer service tested by Quadros et al...

  20. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...plasmids are divided into two sets, each of which includes plasmids with different varieties of tags, both...quickly and easily. These tagged plasmids also include cleavage sites should one need to remove the tag...Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for protein expression in systems... coli. Plasmids designed for these systems may include a signal peptide (SP) that routes the protein through...can improve disulfide bond formation. pCri also includes a special feature for difficult-to-purify membrane...
Showing: 21 - 40 of 628 results