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We narrowed to 43 results for: Neb

Showing: 21 - 40 of 43 results

  4. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...blog High Complexity Golden Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids...

  6. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...questions (besides Addgene, I know both Abcam and NEB have Ph.D.s answering emails and phones). We can’...

  7. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... plasmids with inverted terminal repeats. We use NEB Stable to transform and prep high quality DNA that...

  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...stability, such as some of our viral vectors, in NEB Stable cells. Resource information: This section ...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies...

  11. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are...

  14. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and...

  16. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Overview of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires...resource for planning an NEBuilder HiFi DNA Assembly mix reaction is the NEBuilder Assembly Tool which is...BioBrick® Assembly, Golden Gate Assembly, and NEBuilder® HiFi DNA Assembly. Gibson Assembly allows the...enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New England Biolabs...Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-fidelity...PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of...Generating constructs with multiple point mutations NEBuilder HiFi can simplify multi-position site-directed...

  17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...selected for by manipulating the nebulization time and pressure. Nebulization units, which can be purchased...physical fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening...

  18. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...! #1millionplasmids On to the next million! @CarolineBME @AllostEricSite @LiannaSwanson etc. Missed you...

  20. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...iTOP (induced transduction by osmocytosis and propanebetaine) to describe this method of protein transduction...
Showing: 21 - 40 of 43 results