Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 141 results for: Nut

Showing: 21 - 40 of 141 results

  1. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...showed a rise in transcription in as little as 30 minutes after light stimulation. Under those optogenetic...Second, induction of transcription occurs within minutes of light exposure. Third, the response can be graded...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...obsession with the combination of chocolate and peanut butter), but it’s an undeniably difficult connection...sucrose…..sounds like the gateway to chocolate and peanut butter to me. What’s on the fiber horizon? The...

  5. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...during Zoom lab meetings to chip in ideas and take minutes that can be accessed by anyone at any time. This...simply moves away from the desk to switch off a few minutes. The host could also set aside Breakout Rooms to...

  8. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... regular check-ins with their employees. 15-30 minutes weekly or monthly will usually suffice to keep ...scope” above) or if you can cut it down to thirty minutes. Keep it moving: If you tend to get stuck diving...

  9. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ... meeting on the calendar even if it is just 15 minutes a week. Clearly identify constraints and boundaries...Update frequently with progress reports, meeting minutes and plan changes. Written documentation helps make...

  10. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...experiment (labeling tubes never takes longer than 5 minutes, right?) As a result, I’d always end up stressed... forever grateful, I started setting aside 3-5 minutes to sit quietly at my bench before the start of ...

  11. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...maintained when grown on media containing the missing nutrient. Scientists can exploit these host mutations by...auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless they have incorporated...

  12. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ... RNA-protein interaction.  In as little was one minute BASU generated a strong streptavidin signal (~250...tested at its optimum 18 hour time point, but one minute of labeling with BASU still generated a >30-fold...

  14. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...activation, exhibits stabilized activity for over 30 minutes and can be deactivated using yellow light (590nm...-mcherry-C1-iC++_CA - A slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin...

  15. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...Excuse me, I seem to have lost my place. I need a minute.” I then pulled out my notebook, opened it, found...presenting. The whole process took just under a minute. I can't say the journal club was a rousing success–the...

  16. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand. How ...

  17. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...

  18. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...recombinases.   Figure 1 (modified from Schnutgen et al. Nat Biotech 2003): Basic FLEx switch will...population.    Figure 2 (modified from Schnutgen et al. Nat Biotech 2003): In the presence of Cre...

  19. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...pooled in one tube and ligated in as little as 30 minutes! Alternatively, you might consider Gibson cloning...cut and ligation processes happen in a matter of minutes. Cost: Funding is usually limited - what cloning...

  20. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...air and beyond Turn on the hood for at least 15 minutes before you start any tissue culture work and make... to achieve a consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin...
Showing: 21 - 40 of 141 results