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Showing: 21 - 40 of 225 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...clicking the Download Analysis Data button on the bottom right of the analysis screen. ICE analysis details...representation in the edited pool. The black vertical dotted line represents the cut site, and yellow + symbol...with the red underlined PAM. The vertical black dotted line represents the cut site. Cutting and error-prone...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...in popularity over the last decade. Research by Scott Spicer at the University of Minnesota helps illustrate...about the research is of interest. In his research, Scott Spicer concludes that “… the emergence of the video...numbers." Nature 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals:...

  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount of...antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate a dot plot ...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...products. But it also made SciFi director Ridley Scott wonder what would happen if these corporations would...genetic engineering to exploit human beings. Ridley Scott directed Blade Runner (1982). It is a dystopian ...00)01250-6 Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F (2013) Genome engineering using the ...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... the REC lobe and doubles back on itself at the bottom of the image. Stem loops 1 and 2 extend horizontally...compared to the guide RNA-bound state (PDB ID 4ZT0, bottom), seen from the same angle. Box highlights the ...s41586-022-04470-1 Cofsky, J. C., Soczek, K. M., Knott, G. J., Nogales, E., & Doudna, J. A. (2022). CRISPR-Cas9...

  7. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...) across the 51 nt region using either a top or bottom strand ssODN donor with 40 bp homology arms. The... ssDNA; IDT Megamer® ssDNA Fragments) Test both bottom and top strand ssDNA donors (if possible) The...

  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...AAVrg pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg...114471 AAV5 pAAV-Ef1a-fDIO mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2...

  12. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...expression in forebrain GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5...) pAAV-hSyn-hChR2(H134R)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5...

  15. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441...Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer plasmids... can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter ... to orange) protein. Image courtesy of Roberta Gottlieb. Laker et al., J Biol Chem 2014 Apr 25;289...

  17. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you...and may all your NHEJ mutations be out of frame! Scott Findlay is currently a Postdoctoral Fellow at the...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are sold in packages of ...

  19. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu...robertsonlab/assets/user-content/documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...other proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted...aqueous layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate...
Showing: 21 - 40 of 225 results