...to a target polypeptide. Multimerization in the SunTag system occurs via antibody-peptide labeling; specifically...specifically, cognate peptide epitopes fused to the protein of interest are recognized and thus fluorescently...designed photoswitchable dimers by fusing the SsrA peptide with the light-sensitive LOV2 domain of Avena Sativa...Sativa phototropin 1 (AsLOV2). In the dark, this peptide is caged by the asLOV2 domain and has reduced affinity...reporter proteins fused to the streptavidin-binding peptide (SBP). The addition of biotin disrupts this interaction...

...30370338.  SH3 domain collection reveals novel peptide ligand motifs Article contributed by Michael Lemieux...specificity landscape of the SH3 proteins using peptide-phage display and deep sequencing, revealing that...protein stability, small molecule binding screens, peptide arrays, and phage display selections. In addition...

...subcellular localization peptide. By combining three different localization peptides with four different fluorescent...mixed-subclone system using mStrawberry, localization peptides, and reporters that drive the expression of the...

...light chain regions, connected by a short linker peptide. The lack of constant region makes them less immunogenic...proteins formed by two Fab fragments bound by short peptide linkers. They are bispecific, with a lot of flexibility...a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They...

...amino acids or peptide tags. 5) Strong protein-protein interactions with other peptides. Created with ...strong protein-protein interactions with other peptides, like an Fc-binding domain (Figure 1).  ...

...consists of one substrate (the histone-derived peptide) which can localise the construct specifically ..., which binds selectively to lysine-methylated peptides. This construct is in the middle of a FRET pair...When methylation occurs at the histone-derived peptide, the methyllysine binding domain causes a structural...

... DNA sequence to encode your peptide of interest and produce that peptide in your organism of choice. ...as easy as it sounds. Even for relatively short peptides, there can be many possible ways to encode them...

... via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody (NB) sequence...sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm of...Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed CRISPRi and CRISPRa...

...2019). This technique relies on an S. auerus transpeptidase sortase A variant (mgSrtA), which is capable...monoglycine residue at the N terminus with a small peptide (LPETG). In EXCELL, the mgSrtA variant can be displayed...cell surface proteins with a biotin tagged LPETG peptide. Biotin tagged proteins can then be purified using...

... biology and biotechnology purposes.  Once a polypeptide is biotinylated (more on this below) it can be...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...

... them with peptides that help mediate their transduction, such as the polyarginine peptide described in...iPSCs using a ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2,...

...protein solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds fold properly...designed for these systems may include a signal peptide (SP) that routes the protein through the secretory...

...explain the properties of or draw an amino acid or peptide for some reason. It contains all twenty amino acids...allow you to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, ...

..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ...

...IgG antibody (Figure 1): it is composed of four peptide chains (two identical heavy chains and two identical...recombinantly and engineered to be joined by a linker peptide, resulting in a product called a single-chain Fragment...

...of VP64 per each dCas9, SunTag uses a repeating peptide array to fused with multiple copies of VP64. By...two-component system dependent on gRNA design (SAM) or peptide design (SunTag). This streamlines its delivery,...

... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...recombinant proteins with a naturally secreted plant peptide allows for easier access and purification of a ...

...short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins in cells (Figure...covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine...

...neuronal circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI...simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-...

...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP...