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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 84 results for: PEP

Showing: 21 - 40 of 84 results

  1. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...synthesis Bactericidal 100 ug/mL Bleomycin glycopeptide Induces DNA breaks Bactericidal 5-100 ug/mL...Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide Alters outer membrane permeability Bactericidal...Chloramphenicol   Binds 50S ribosomal subunit; inhibits peptidyl translocation Bacteriostatic 5-25 ug/mL in EtOH...

  2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...30370338.  SH3 domain collection reveals novel peptide ligand motifs Article contributed by Michael Lemieux...specificity landscape of the SH3 proteins using peptide-phage display and deep sequencing, revealing that...protein stability, small molecule binding screens, peptide arrays, and phage display selections. In addition...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...subcellular localization peptide. By combining three different localization peptides with four different fluorescent...mixed-subclone system using mStrawberry, localization peptides, and reporters that drive the expression of the...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...amino acids or peptide tags. 5) Strong protein-protein interactions with other peptides. Created with ...strong protein-protein interactions with other peptides, like an Fc-binding domain (Figure 1).  ...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...light chain regions, connected by a short linker peptide. The lack of constant region makes them less immunogenic...proteins formed by two Fab fragments bound by short peptide linkers. They are bispecific, with a lot of flexibility...a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...consists of one substrate (the histone-derived peptide) which can localise the construct specifically ..., which binds selectively to lysine-methylated peptides. This construct is in the middle of a FRET pair...When methylation occurs at the histone-derived peptide, the methyllysine binding domain causes a structural...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody (NB) sequence...sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm of...Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed CRISPRi and CRISPRa...

  8. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... DNA sequence to encode your peptide of interest and produce that peptide in your organism of choice. ...as easy as it sounds. Even for relatively short peptides, there can be many possible ways to encode them...

  9. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...2019). This technique relies on an S. auerus transpeptidase sortase A variant (mgSrtA), which is capable...monoglycine residue at the N terminus with a small peptide (LPETG). In EXCELL, the mgSrtA variant can be displayed...cell surface proteins with a biotin tagged LPETG peptide. Biotin tagged proteins can then be purified using...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... biology and biotechnology purposes.  Once a polypeptide is biotinylated (more on this below) it can be...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...

  11. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... them with peptides that help mediate their transduction, such as the polyarginine peptide described in...iPSCs using a ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2,...

  12. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...protein solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds fold properly...designed for these systems may include a signal peptide (SP) that routes the protein through the secretory...

  13. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...explain the properties of or draw an amino acid or peptide for some reason. It contains all twenty amino acids...allow you to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, ...

  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ...

  15. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...beetle (Pyrophorus plagiophthalamus), deep-sea copepod (Gaussia princeps), Japanese firefly (Luciola cruciata...or naphthyl-luciferin analogs ATP No Deep sea copepod (Gaussia princeps) Natural 480 20 Coelenterazine...

  16. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...IgG antibody (Figure 1): it is composed of four peptide chains (two identical heavy chains and two identical...recombinantly and engineered to be joined by a linker peptide, resulting in a product called a single-chain Fragment...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...recombinant proteins with a naturally secreted plant peptide allows for easier access and purification of a ...

  19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins in cells (Figure...covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...neuronal circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI...simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-...
Showing: 21 - 40 of 84 results