We narrowed to 127 results for: PIR

1. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

   Type

   Blog Post

   Published

   Sept. 15, 2014, 6:05 p.m.

   ...the presence of scientific heroines is always inspiring. I was especially excited to attend this event...genome. Their award acceptance addresses were inspiring! We also got to hear more from them during a panel...

2. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

   Type

   Blog Post

   Published

   June 19, 2018, 1:38 p.m.

   ...bacteria and archaea to regulate their buoyancy. The Shapiro lab recently discovered that gas vesicles also ...Lakshmanan, A., Farhadi, A., Kumar, S.R., Nety, S.P., & Shapiro, M.G. (2018). Acoustic reporter genes for noninvasive...

3. Cultivating Community Science at BosLab

   Type

   Blog Post

   Published

   Nov. 2, 2017, 1:35 p.m.

   ...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

4. With Our New Viral Service, We're Taking CRISPR Further!

   Type

   Blog Post

   Published

   Dec. 6, 2016, 3:30 p.m.

   ...researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory...Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs...

5. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ... a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic reporter gene ...water from entering their hollow interiors. The Shapiro lab discovered in 2014 that these gas vesicle proteins...

6. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...

7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   Published

   March 8, 2017, 3:15 p.m.

   ...your plasmids in: DH5α, NEB Stable, ccdB Survival, Pir1, or other. Whenever possible, we propagate plasmids...contain the R6Kγ origin of replication, choose the Pir1 strain. If your plasmid cannot be propagated in ...

8. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...stimulation parameters must also be determined empirically but it’s best to try those that have already ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

9. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

   Type

   Blog Post

   Published

   June 5, 2014, 3:32 p.m.

   ...doing this with very little money. So when did inspiration strike us so hard that we upended our lives and...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...

10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...female scientists whose tweets I find really inspirational. They share experiences, articles about women...

11. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    Published

    July 1, 2025, 1:15 p.m.

    ... just be sure to check that your RNase has not expired (or been forgotten).    Improving read accuracy...purification protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...to the N- and C-terminal end of each PIP and empirically test which combination provides the best fluorescent...constructing appropriate controls may requires that you empirically test a number of mutants to identify those that...

13. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

14. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ...researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic...researcher at the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a ...

15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ...interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously in many cell ...

16. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...a stupendous listener, but I aspire to  be. If you don’t at least aspire to be a good listener and consciously...

17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...supported, and can more fully identify with and be inspired by the mentor’s successful example. For evidence...2014-15, University of Valencia): “It is very inspiring to be in contact with mentors who are at a professional...

18. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...relatives suffering from similar diseases. Others were inspired by the imminent threat of climate change to study...interesting questions and your excitement will in turn inspire the people around you. Be proactive Rather than...

19. 'Tis the Season to #DeckTheLab

    Type

    Blog Post

    Published

    Dec. 2, 2016, 3:30 p.m.

    ...Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. ...

20. Deck the Lab 2022!

    Type

    Blog Post

    Published

    Jan. 17, 2023, 2:15 p.m.

    ... The lab has officially welcomed the Christmas spirit. It's just unfortunate that we had no choice but...