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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 118 results for: PTS

Showing: 21 - 40 of 118 results

  1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...created a video to help scientists visualize the concepts discussed in the paper. The transcriptional control...behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors often fall...

  2. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating...cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent...

  4. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ... protein (orange). A low imidazole wash buffer disrupts the weak interaction of the contaminating protein...protein of interest. A high imidazole elution buffer disrupts the interaction of the protein of interest and...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... (Fig. 1). They first copy themselves as RNA transcripts and then rely on a reverse transcriptase enzyme...mechanism. First they copy themselves as RNA transcripts and then use their reverse transcriptase (RT)...

  10. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...RAS signaling pathway and includes 180 unique transcripts. Each gene was chosen to represent the primary...variant in 38 types of human cancer. Many of the transcripts are not available from other existing sources...

  12. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ... on TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question...

  13. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...

  19. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ... cortical neurons, far out-performing previous attempts to engineer potassium-selective channelrhodopsins...
Showing: 21 - 40 of 118 results