We narrowed to 123 results for: Pts

...created a video to help scientists visualize the concepts discussed in the paper. The transcriptional control...behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors often fall...

...round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating...cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent...

...sciences were called into question after credible attempts to replicate major findings could not be replicated...difficult to replicate. Unclear methods Until someone attempts to replicate the results of another’s work, it...

... protein (orange). A low imidazole wash buffer disrupts the weak interaction of the contaminating protein...protein of interest. A high imidazole elution buffer disrupts the interaction of the protein of interest and...

...the temporal and spatial localization of mRNA transcripts in tissue. This is useful for labeling specific...results in an observable “sidewinder” phenotype that disrupts wildtype locomotion.   Create your own plasmids...

...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

...first site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology...

...therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility for preclinical...similarly low rates of reproducibility. Failed attempts to apply irreproducible results to future work...

...carried by extracellular vesicles (EVs), edited transcripts could endow neighboring cells with pro-oncogenic...system of dynamic and redundant roles. This makes attempts to purify or overexpress proteins inappropriate...

... (Fig. 1). They first copy themselves as RNA transcripts and then rely on a reverse transcriptase enzyme...mechanism. First they copy themselves as RNA transcripts and then use their reverse transcriptase (RT)...

... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...

...RAS signaling pathway and includes 180 unique transcripts. Each gene was chosen to represent the primary...variant in 38 types of human cancer. Many of the transcripts are not available from other existing sources...

... on TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question...

...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...

...Any (see notes) Any Data repository Only accepts data for antibodies in Addgene’s repository  ...

...with introductory lectures targeting three broad concepts: the genetic basis of disease, cancer, and viral...

... Brilliant technologies adopt basic biological concepts and employ them in creative and innovative ways...-binding peptide (SBP). The addition of biotin disrupts this interaction and thus triggers a synchronous...

...growth until eventually the patient loses mobility. Attempts to remove excess bone surgically cause the body...

...your science, whether it’s in presentations, manuscripts, or social media. If you want to draw your own...

...site to cleave and inactivate any read-through transcripts of the effector gene. Effector genes, any gene...