We narrowed to 56 results for: RHO
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TypeBlog PostPublishedAug. 31, 2023, 1 p.m.... by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....Gottschalk lab has surveyed a range of microbial rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis...membranes, which might beneficially influence the rhodopsin properties. Find these voltage biosensors at Addgene... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5. Get the channelrhodopsin here Astrocyte specific tight affinity glutamate... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center. ...Many thanks to our guest blogger Patrick Miller-Rhodes from the University of Rochester Medical Center... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....Activation of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from...mouse to deliver Cre-dependent expression of Channelrhodopsin 2 (ChR2), a modified receptor protein that... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....://doi.org/10.1038/s41586-025-08877-4. Two powerhouse tools combine for dynamic protein labeling By...to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....and mitophagy, can be tracked using dyes like Rhodamine 123 (R123) (Liu et al., 2017). In multi-color ...diffraction limit of light. Dyes like silicon-rhodamine or Janelia Fluor (JF) dyes have specific properties... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m....Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are...Vidal-Sanz M., and Aguayo A.J. (1987). The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and ... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single...expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv, et al. Nat Methods. 2016. PubMed... -
New Viral Vectors - Spring 2025
TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato ... -
Newly Updated AAV Data Hub!
TypeBlog PostPublishedFeb. 7, 2023, 2:15 p.m....report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....containing plasmids for expressing different types of rhodopsins and sensors as well as references and links to... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m...."Optical control of zebrafish behavior with halorhodopsin." Proceedings of the National Academy of Sciences... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....trophies will serve as badges of honor for our powerhouse depositors who have generously shared their reagents... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m.... channelrhodopsin... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....spectrum. I recently presented Kurt Vonnegut’s Slaughterhouse-Five, and the responses ranged from “one of... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....Shi L, Netland J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal ... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....Cas9’. The Cas9 nuclease is a genome engineering powerhouse, and for good reason. It is guided by an RNA ...