We narrowed to 41 results for: RIK
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TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....requested dozens of times in 2 weeks. This is a striking example of the contributions that scientists are...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098. LipoGlo... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....CRISPR editing. Conclusions & implications One striking feature of this paper is the failure of the embryos... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....on the dimerization tendency in living cells. Strikingly, the OSER assay reveals that tagRFP does not ... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m.... to genome instability. Thus, it’s important to strike a balance between productive levels of R-loops ... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....Central PMCID: PMC2238901. Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....although both are of course preferred! When trying to strike that balance, you may wonder: how close is close... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....when we needed additional expertise. Perhaps most striking is that there were seven institutions involved... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....with very little money. So when did inspiration strike us so hard that we upended our lives and took this... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m.... all possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, ... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....that UV light only kills microorganisms that it strikes directly. UV light exposure is also harmful for... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital PCR as a tool for titration... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....DNA replication and regulation Plasmids must also strike a balance: replicating too slowly will eventually... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m.... right now to capture interesting key results, striking images, creative ideas, and engaging stories as... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction and expression of genes for metabolic... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....2013): 34-38. PubMed PMID: 23142428. 17. Hamada, Mariko, et al. "Development of a ligase detection reaction... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....these improvements from the Mello lab resulted in strikingly high editing efficiencies, obtaining as many ... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....https://doi.org/10.1021/acssynbio.5b00124. Obst, Ulrike, et al. “A Modular Toolkit for Generating Pichia...