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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 35 results for: RNAi

Showing: 21 - 35 of 35 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...shRNA is used as a negative control for mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO...

  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... & CRUSH – rapid & conditional gene silencing in RNAi transgenic mice The Jackson-Grusby lab has designed... in conditional and reversible gene silencing in RNAi transgenic mouse models and embryonic stem (ES) ...transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which Cre expression causes...

  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...targeting or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate...

  8. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...expected protein. Common approaches include using RNAi to knock-down expression of your target or CRISPR...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check ...

  11. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's...their loop region. After infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs...the gRNAs and their iBAR sequences. Since the sgRNAiBAR library covers every annotated human gene, it ...number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome Biology, 2019. PubMed...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...can be used to detect specific phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can...28533371. PubMed Central PMCID: PMC5468623. 11. Várnai, Péter, and Tamas Balla. "Live cell imaging of ...
Showing: 21 - 35 of 35 results