We narrowed to 41 results for: RPA;

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...genes of some human cancers from their normal counterparts. Nature 301, 89–92. https://doi.org/10.1038/...

2. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ...its guide RNAs are smaller than their SpCas9 counterparts, so they will be easier to deliver in low-capacity...

3. Common Injection Routes in Mice

   Type

   Blog Post

   Published

   Jan. 22, 2025, 9:42 p.m.

   ...instillation is very similar to its intratracheal counterpart, as both procedures target the lungs. The main...

4. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   Published

   Jan. 30, 2024, 2:15 p.m.

   ...have kinetics comparable to its one component counterpart and is significantly brighter and available with...

5. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   Published

   Feb. 23, 2016, 3:30 p.m.

   ... see the linked blog posts. Compared to its counterparts NHEJ and HDR, MMEJ doesn’t get a lot of press...

6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons by the...

7. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...ve got amp? Well, amp is less stable than its counterparts and is also known to produce satellite colonies...

8. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...mechanisms, only one of the two strands’ DNA counterparts from step 2 will be integrated by the viral ...

9. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...more planning and finesse then their knock-out counterparts. To kick off a knock-in experiment the first...

10. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...its guide RNAs are smaller than their SpCas9 counterparts, they will also be easier to deliver in low-...

11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...depleted at greater rates than their non-targeting counterparts, suggesting that safe-targeting guides are more...

12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...has much lower efficiency than its wild-type counterpart.  In search of a smaller, but equally potent ...

13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ...revealing that about 70% of human genes have a counterpart in zebrafish. Understanding the function of these...

14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...important advantages over their amino acid-based counterparts, antibodies and enzymes, such as: Design and...

15. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...mirrors the complexity of understanding their wild counterparts. Those familiar with viral vectors, and especially...

16. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    Published

    Sept. 29, 2020, 1:15 p.m.

    ...that nearly all disease-linked human genes have counterparts in the rat (Gibbs, et al., 2004). Additionally...

17. Five Popular Model Organisms

    Type

    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ...chromosomes and about 23% of yeast genes have a counterpart in humans (Liu et al., 2017) . Thus yeast can...

18. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...generally more toxic to cells than their plasmid counterparts. Another consideration for dsDNA templates is...

19. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...Proximity ligation tools like TurboID and its mini counterpart label nearby proteins with biotin. The biotinylated...

20. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...background activity and activation as their wild-type counterparts. The Janovjak lab has deposited their 3 Opto-RTK...