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We narrowed to 186 results for: Rras

Showing: 21 - 40 of 186 results

  1. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...produce functional R-mAbs. It was recognized that an aberrant IgG light chain transcript was expressed by some...the restriction enzyme BciVI was present in the aberrant light chain sequence, but was predicted to occur..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG ...

  2. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...per each dCas9, SunTag uses a repeating peptide array to fused with multiple copies of VP64. By having... histone acetyltransferase domain capable of rearranging chromatin structure. This method has shown higher...Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation...

  3. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering them to these...start from scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, ...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...transcription) is demarcated by viral genome replication (Harrach, 2014). Given that the early genes encode proteins...10.1080/22221751.2019.1690953. PMID: 31749409. Harrach, B. (2014). Adenoviruses: General Features. In ... Zhou, D., Lin, S. W., Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl...

  5. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ... the past few years, scientists have created an array of base editing reporters that can help you do just...successfully used GO to induce the translation of an array of different reporters including mScarlet-I, luciferase...initiate protein expression, it can be used for an array of detectable proteins including fluorescent proteins...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...2009). Rodents have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016...biology tools to generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed...in research (Krämer, 2015). As a result, a vast array of molecular tools, such as gene manipulation and...

  7. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...so that at least one might be useful in a broad array of pathogenic Proteobacteria. We placed the toxin..., Shigella flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions...development of allelic exchange vectors will produce an array of great options, and continue to improve the efficiency...

  8. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... has become an ideal model organism to study an array of topics including development, genetics, and the... A huge advantage of using the fruit fly is the array of genetic tools, such as the GAL4/UAS and LexA ...organisms. Worms have been used to investigate an array of biological systems and processes including the...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11...reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12...be used to visualize translocations, genome rearrangements and cancer-associated chromosome shattering...subpopulation of mitochondria is selectively labeled by irradiating the photoactivatable FP, then the overlap between... MYC, Flag), they can be deleterious and cause aberrant protein folding, expression, or even affect cell...

  10. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ... depositors for sharing your science with us! Corralling and Cornering the Goldilocks Antibody: Guest ...drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen...

  12. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...supporting your research, Addgene is creating an array of educational materials to accompany our antibody..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of...

  15. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your work The part...programmed to appreciate and understand. We build narratives around the self, too — when talking to friends...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well ...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

  18. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...guides require two arrays for Cas9, two guides can be streamlined into a single array for Cas12a. Image...

  19. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...neurotransmission. These receptors can be activated by an array of different ligands, including hormones, neurotransmitters... showing no constitutive activity. There are an array of DREADDs that activate different G proteins, work...

  20. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... from the Caltech lab of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription...
Showing: 21 - 40 of 186 results