We narrowed to 63 results for: SHR
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TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....they tested truncated ADARDDs to see if they could shrink the construct. They identified ADAR2DD(delta984...Although REPAIRv1 knockdown is more precise than shRNA knockdown, it still displays substantial off-target...
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CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m.... size distribution. When comparing LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown...a distinct contrast to the hundreds of observed shRNA off-targets. They subsequently used catalytically... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia et al., 2016). Despite the...10.1038/nbt.2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation. Nature 532:293–293 . https... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc... Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog PostPublishedOct. 2, 2025, 1:15 p.m.... Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ...Alghamdi, R. A., Tomancak, P., Reynaud, E. G., & Shroff, H. (2017). Assessing phototoxicity in live fluorescence... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m.... knockdown, consider using shRNA vectors instead. With both siRNA and shRNA, it can be difficult to achieve... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m....Mario, dressed as Santa, delivering gifts to the Mushroom Kingdom. Kravitz Lab 2019 Holiday Door is up ... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela Abitua Thanks... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....Dolly, Eddy, Emma, Eric, Gizela, Henry, Ina, Inna, Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine,... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the phenotype is without... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m.... we also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely used genome editing... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....endogenous RNA imaging tool. Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head for gene expression... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....response has been a longstanding challenge. Using shRNA, TALEN, or ZFN to edit immune cells poses challenges... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by... -
Viral Vectors 101: Viral Vector Elements
TypeBlog PostPublishedJuly 25, 2023, 1 p.m....construct, to cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....voltage indicator Archon Article contributed by Shreya Vedantam Listen to the voltage indicator podcast... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....beginning Let’s take a quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr...