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...family, he did not seem much different than my second son of similar age. When he came to visit us and watch...  Many thanks to our guest blogger Kurt Swanson! Kurt Swanson is a structural biologist and protein engineer...This post was contributed by Kurt Swanson a structural biologist and protein engineer currently working...

... a mCherry OptoNB to the catalytic domain of the Sons of Sevenless (SOS), a guanine nucleotide exchange...

...Transposagen What is the piggyBac™ transposon? The piggyBac™ transposon was originally identified in moths...until 2005 [1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The ...titrating the transposase to transposon ratios. High ratios of transposase to transposon result in a greater number...ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source ...you to quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences distributed...genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful...transposase facilitates the integration of the transposon specifically at ‘TTAA’ sites randomly dispersed...

...important for social and regulatory reasons. 1.) The transposon consists of a mirrored set of inverted....   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is one such system...Transposons are sequences of DNA that can move around in a genome. In a laboratory setting, transposons...the site of insertion. In both of these cases, transposons combine the advantages of viruses and naked DNA...mechanisms. The use of non-viral vectors, like transposons, avoids many, though not all, of these defenses...reaction occurs which cuts the DNA.3.) The released transposon can now bind a strand of DNA with a TA dinucleotide...plasmid is empty following the removal of the transposon; the plasmid is then degraded by the cell.4.)...

...9d0855a1b533" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Visit Addgene's Data Hub"{% ...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"border-radius":"10px","padding-left...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute...end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}{"no_follow":false,"open_in_new_tab...

...recruits other silencing proteins. Figure 1: Comparison of shRNA and miRNA processing and silencing pathways...genomic sequences (Figure 2B).   Figure 2: A comparison of an shRNA plasmid sequence and an miRNA plasmid...14305620e565" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Browse Addgene's shRNA plasmid...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"border-radius":"10px","padding-left...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...

...fcd590559fc0" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Browse the Allen Institute's...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"border-radius":"10px","border-style...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute...end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}{"no_follow":false,"open_in_new_tab...

...a7049139e11d" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Find OCaMP plasmids available...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"padding-left":"200px","padding-right...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute...end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}{"no_follow":false,"open_in_new_tab...

...but he or she probably has reasons for it. Do not take criticism personally or get defensive. Look at ...important issues in person or over the telephone instead of email. As you cannot see a person’s body language...get nods from nearly every participant. Robert Bramson, author “Coping with Difficult People,” identified...specific strategies to deal with each of these personality types is beyond the scope of this article, there...resolve conflicts What Is Assertiveness? An assertive person is able to communicate their ideas confidently ...your supervisor, even if he or she is a difficult person: 1) Understand your supervisor’s expectations ...or she would like a written report or to meet in person.   Regardless of your supervisor’s management style...

...were usually the products of teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I ...intelligent, motivated person really could know a little bit about everything or in Jefferson’s case, even a lot...work of more than one person. A selfish part of you might not want another person’s name on your next paper...clear why that person is there. If it becomes clear after a few meetings that a person is neither adding...all our externally facing kit pages. No single person could possibly understand every nuance of how kits...should have a goal, or at the very least, a clear reason for existing. Here are a few types of teams that... - with the possible exception of when Thomas Jefferson dined alone.” There was a time in human history...

...to the conclusion that a conflict is personal (”Person A and Person B just don’t get along”) when in fact...conflict are substantive (“Person C told Person A one thing and inadvertently told Person B something else.")...also be categorized as either “personal” or “substantive”. A personality-based conflict occurs when two...multiple channels is not reasonable or sustainable. That said, when you know that a person or team should be ...large organization, because confirming that every person has read and understood every communication that...assign the responsibility in question to a specific person or team. What Makes You a Good Conflict Resolver... for a fight. But you also can’t be the type of person who avoids conflict at all costs or actively runs...

... result of the necessary restrictions on close personal contacts. However, that should not deter any student... letter can be found on the Addgene blog.   3. Personal statement brilliance In addition to sending in... cover letter, you are always asked to draft a personal statement, which is the perfect opportunity to...questions, thus devoting some time to writing a solid personal statement will pay dividends. This is the only...and forward-looking. Embrace it, and let your personality leap out of the page. Finally, as previously ...find the tools that can help you craft a solid personal statement.  4. Letters of recommendation wisdom...to vouch for the character you painted in your personal statement. Make certain to choose your recommenders...

... like we finish writing about one dual CRISPR-transposon system before another exciting new advance emerges...combined with the large sequence capacity of transposons open whole new worlds to explore. The newest ...bacteria. In the course of evolution, Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas...protein recruits transposition cofactors. The transposon proteins integrate a payload sequence into genomic...Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large sequences into...labs. Evolving CASTs for new contexts Natural transposons evolve under contrary selection pressures: high...researchers suspected natural evolution would produce transposons with sub-maximal efficiency, and this barrier...

... year’s Michael Davidson and Roger Tsien Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger...variation at large scales and making personalized medicine a reality,” Chiasson explains. Elizabeth Unger: Developing...The late Michael Davidson and Roger Tsien played enormous roles in fluorescent protein development by...To honor their legacy, we launched the Michael Davidson and Roger Tsien Commemorative Travel Awards in...Unger, and Michal Bernach. Melissa Chiasson: Quantifying the abundance of thousands of protein variants ...time is time consuming and unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler's lab at...from each bin are then extracted and sequenced. Chiasson is currently using VAMP-seq to study vitamin K...

... A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R., & Adamson, B. (2024). Improving prime editing... Chen, C., Nelson, J. W., Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu,.... coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small size comes at... even in contexts where expression was reduced (Nelson et al., 2022). PE7: An alternate approach to RNA...RNA stability This recent contribution by the Adamson lab also addresses the problem of RNA stability....References Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M....A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., & Liu, D. R. (2019...

...fused to a shortened R2Tocc transposon protein, and can "retarget" the transposon to new loci in the genome...Image courtesy of IPI.   Repurposing retrotransposons for DNA editing By Emily P. Bentley For RNA-guided...beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the pithy name "site-specific target-primed...this tool based on several new site-specific retrotransposon elements they identified and characterized ...characterized (Fell et al., 2025). In retrotransposon insertion, a combined endonuclease-reverse transcriptase uses...genome. The modified transposon RNA has untranslated regions (U, blue) on the outside of the homology arms...(teal). The Cas9 gRNA targeting guide and the transposon RNA homology both help direct the fusion construct...

... A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R., & Adamson, B. (2024). Improving prime editing... Chen, C., Nelson, J. W., Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu,...-RNA base pairing with the primer binding site (Nelson et al., 2022). PE7: This editor includes the fused...A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., & Liu, D. R. (2019...e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette,...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...

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...new variant was named ChrimsonR. Plasmids containing Chronos, Chrimson, ChrimsonR, CsChR, and CoChR have...inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al., Dev Cell...Updated mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab...selecting the important elements from existing transposon and vector systems and creating an all-synthetic...British idiom for “taking by surprise”) by Margaret Robinson’s lab, and more recently used by Stephen Royle’...Shining a light on channelrhodopsins – Chronos & Chrimson Through de novo sequencing of 127 algal transcriptomes...include a pair of channelrhodopsins, Chronos and Chrimson, which have enabled the group to perform independent...

...everyone wins. What are the underlying reasons for this person’s request? Your colleague is if someone...need help from another person/team at the company. How do I negotiate with the person/team for help? What...can help you understand the perspective of all persons involved and be used as a guide to point you toward...important plans? What is most important to each person? Someone has to be the first author but another...agrees to pick up both children while the other person uses an available after-hours time slot.  You were...thoughtful about what is most important to each person. It can especially be difficult to think creatively...supervisors and lead to you being remembered as a person who tries to find solutions that benefit everyone...