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We narrowed to 23 results for: TOP-GFP reporter element

Showing: 21 - 23 of 23 results
  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... (Dlx5/6) enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating... developed a new AAV approach using regulatory elements that restrict expression to this cell type. To...reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 Listen to the podcast...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...
  2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to increase transgene protein... translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...
  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Reports, 34(13... control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, ...fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of the systemic...frequently been used for screening candidate regulatory elements such as enhancers and promoters for cell type-...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer...
Showing: 21 - 23 of 23 results