We narrowed to 22 results for: TOP-GFP reporter element

1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to increase transgene protein... translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...

2. Viral Vectors 101: Systemic Capsids

   Type

   Blog Post

   Published

   Oct. 12, 2023, 1:15 p.m.

   ...enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Reports, 34(13... control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, ...fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of the systemic...frequently been used for screening candidate regulatory elements such as enhancers and promoters for cell type-...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer...