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We narrowed to 867 results for: TOR

Showing: 21 - 40 of 867 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...searched for and eventually identified a postdoctoral mentor. A good mentor typically writes the grants that ... of reference- the graduate school mentor, the postdoctoral mentor, and at least one more person that ...was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia Research ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... of your postdoctoral training experience. If you're going to commit to doing a postdoctoral fellowship... you how to write as well as mentor. Yet, even the most dedicated mentor is not always available for mentees...different roles? A second or third mentor. Therefore, seek out additional mentors. I found this beneficial for...

  2. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... as a shuttle vector? Are viral vectors shuttle vectors? The production of viral vectors typically involves...their vectors in fast-growing bacteria first. In fact, most yeast vectors and many mammalian vectors are...shuttle vectors are involved in the production of viral vectors, we consider the viral vectors themselves...between species. Figure 1: Shuttle vectors are vectors that can function in more than one host species...Not quite. Shuttle vectors have to address several big differences in the ways vectors are used across species...needed to retain the vector as cells divide. In transient transfection, the vector remains episomal, meaning...of the vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides...

  3. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...are doing is telling a (nonfiction) story. For there to be a story, you need to have conflict. There are...complete your story: an introduction, and a resolution.   Fig. 2: The structure of a story - not just...communication science. Science communicators also use the hero’s journey, stories from patients or other affected...unrelated stories that they then tuck the science into. There are almost limitless storytelling options,...pursuit of achieving knowledge. Or, as we in the storytelling business like to say, science is all about navigating...inherent tension, is interesting. It’s what drives a story forward - and this drive is so powerful that it ... communications, but they’re firmly part of the story to the scientists doing the work.  And while these...

  4. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...use viral vectors for many experimental purposes. To help you make sense of all the viral vector information...Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check out our ...our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the Image Above ... viral vector...

  5. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ... Read All of Our Editor's Choice Blog Posts Last month there was plenty of excitement at Addgene. Our...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...Grad School Advice Part 2: Building Community  My editor’s choice pick for this month is “Grad School Advice... editor's choice...

  6. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... Read All of Our Editor's Choice Blog Posts As I’m sitting in the San Francisco International Airport...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer...for FOP, Running for AJ I have two picks for the editor’s choice posts this month. The first, "Technique...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ... editor's choice...

  7. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...In response to a bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...by PCR & Single Base Editing with CRISPR For my editor’s choice picks this month, I’m picking two posts...give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend taking a look...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

  8. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...each and every month, we've decided to start an "Editor's Choice" series. Each month, I'll summarize the...on topics ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it...I love all of our blog posts, my pick for the "editor's choice" post this month is from guest blogger ... editor's choice...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...future) applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s...design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned to...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications,...viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The vectors could...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more...Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly popular in the lab...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses for FLEx...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo Sabatini Wenbiao Chen Liquin...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...due to factors such as the experimental cell type and the specific genes cloned into the vector.3  2A ... the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to...get started with them. Why Use multicistronic vectors? Detecting cells that are expressing your gene,...expressing your gene of interest at high levels. Unlike vectors that express screenable or selectable markers from... the same transcript. Of course multicistronic vectors do not have to exclusively be used as a means of...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...using synthetic vectors and cultured mammalian cells. Of course, these mammalian vectors must be compatible...Instead, reporter genes are often employed to easily monitor transfection efficiencies and expression levels... mammalian vectors...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...typically found as a single copy. Unlike the ARS vectors, CEN vectors are stable without integration. Yeast Episomal...plasmid maintenance within an E. coli cell, but vectors can be widely utilized across many different cell...core replication and resistance features of yeast vectors and explain how they differ from the bacterial ...previously described. Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex ... and yeast cells. To be useful in the lab, the vectors must contain a yeast-specific origin of replication...replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed...

  14. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...Science Mentors series: Part 1: "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring ...choose an advisor/principal investigator (PI) that will also serve as a good mentor. To read more about what...choosing a lab for postdoctoral work. However, selecting a lab with a PI who is a good mentor is important ...to learn about her mentoring style. How can I find out if an advisor is a good mentor? You have to ask ...on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s focus on how to ...what makes a good mentor, see the previous post in this series. Check out Joanne's Reddit AMA Different...Different types of mentors for grad school vs. postdoc Choosing an advisor to complete a successful PhD is different...

  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...recall, are the outer shell of an AAV vector, which determine the vector’s tropism, including its ability to...payloads. Since most systemic vectors are primarily derived from AAV9, the vectors have to be administered ...Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo designs and... CNS (broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) ...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown ...on the Addgene blog Viral Vectors 101: AAV Variables That Matter Viral Vectors 101: An Introduction to ...). Targeting AAV vectors to the central nervous system by engineering capsid–receptor interactions that...

  16. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...jGCaMP7 and jGCaMP8.   Viral vectors So, what do viral vectors have to do with all of these cool ...also already packaged into viral vectors and ready to go. Pro tip: Vectors with ready-to-use AAV preparations... a calcium sensor Genetically encoded calcium indicators (GECIs) are fluorescent proteins linked to calcium...troponin C). Their fluorescent readouts are used to monitor calcium concentrations and transients in living...living cells and organisms. Unlike chemical indicators, these DNA constructs can be delivered to intact tissues...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral...would not be great for a gene therapy vector! For viral vectors, only the protein for reverse transcriptase...components of the vector particle, some of the RT protein gets packaged inside of the vector particle, along...and this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is ...Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of Gene-Editing Systems...seem like an unlikely candidate to use as a viral vector, but retroviruses have been developed into some...gamma-retroviruses — that have been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses...

  18. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...Bicistronic vectors combine excitation and inhibition by using both excitatory and inhibitory channels activated...BioRender.com.   Opto-G-protein coupled receptors  G-protein coupled receptors (GPCR) regulate cellular function...some broad classes of optogenetic tools, and what factors to consider when choosing one.     Figure...light-gated ion channels, optogenetic G-protein coupled receptors (OptoXRs), and photoswitches. Light-gated ion ...stimulation to either activate or inhibit a specific receptor or pathway.  Light-gated ion channels Light activated...light within a specific range of wavelengths. Excitatory channels allow positively charged ions such as...gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are usually light-driven pumps that pass...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing... contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular...cellular function, this would also be a higher risk vector. While it is unlikely that you, or anyone else ... you can receive appropriate treatment and/or monitoring. Virus-specific concerns   In nature, some viruses..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-...

  20. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101:...heterologous envelope or a post-transcriptional regulatory element can be used for either type of retrovirus...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...strains have been engineered to induce a lower inflammatory response within hosts. Relative to the other... Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. ...
Showing: 21 - 40 of 867 results