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We narrowed to 867 results for: TOR

Showing: 21 - 40 of 867 results

  1. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...method for targeting receptor expression as a ligand and receptor pair. Targeting Receptor Expression Using... translation to human patients. Picking receptors The receptor you choose is largely dependent on what... designer receptors exclusively activated by designer drugs, are G protein-coupled receptors (GPCRs), ...derived from muscarinic receptors. The most popular agonist for muscarinic receptor derived DREADDs has been...pharmacologically selective actuator modules, were developed with the goal of creating receptors with less endogenous...on the Addgene blog New to viral vectors? Browse our Viral Vectors 101 blog posts Read blog posts about... relies on genetically introducing engineered receptors that are only activated by otherwise inert drugs...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...specifically recognized by another factor, the receptor. The receptor could be something like an antibody...for multiplexed imaging for advanced monitoring of different factors, in different or the same cellular ...wide-range of uses to monitor the fundamental cellular building blocks. Physiological factors Unlike the previously...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...directly proportionate to the amount of a given factor within a biological system. Basically, biosensors...antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte...analyte. This signal depends on the system and the receptor, but readouts could involve fluorescence, pH change...

  3. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...of the Fc receptors FcγRIIB1 and FcγRIIB, inhibit) specific immune responses. These receptors are specific...so IgG isotypes bind to Fcγ receptors; IgE isotypes bind to Fcε receptors; and so on and so forth. There...of different receptors, but their downstream effects can be sorted into three main effector functions.  ... about Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound... RA, Towers TL, Presta LG, Ravetch JV. Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor...antibody's binding affinity (Janda et al., 2016; Torres & Casadevall, 2008) but…does it really do anything... the innate immune response, by binding to Fc receptors on other immune cells. (psst! Need a quick review...

  4. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...use viral vectors for many experimental purposes. To help you make sense of all the viral vector information...Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check out our ...our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the Image Above ... viral vector...

  5. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ... Read All of Our Editor's Choice Blog Posts Last month there was plenty of excitement at Addgene. Our...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...Grad School Advice Part 2: Building Community  My editor’s choice pick for this month is “Grad School Advice... editor's choice...

  6. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... Read All of Our Editor's Choice Blog Posts As I’m sitting in the San Francisco International Airport...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer...for FOP, Running for AJ I have two picks for the editor’s choice posts this month. The first, "Technique...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ... editor's choice...

  7. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...In response to a bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...by PCR & Single Base Editing with CRISPR For my editor’s choice picks this month, I’m picking two posts...give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend taking a look...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

  8. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...each and every month, we've decided to start an "Editor's Choice" series. Each month, I'll summarize the...on topics ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it...I love all of our blog posts, my pick for the "editor's choice" post this month is from guest blogger ... editor's choice...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...future) applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s...design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned to...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications,...viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The vectors could...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more...Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly popular in the lab...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses for FLEx...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo Sabatini Wenbiao Chen Liquin...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...due to factors such as the experimental cell type and the specific genes cloned into the vector.3  2A ... the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to...get started with them. Why Use multicistronic vectors? Detecting cells that are expressing your gene,...expressing your gene of interest at high levels. Unlike vectors that express screenable or selectable markers from... the same transcript. Of course multicistronic vectors do not have to exclusively be used as a means of...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...using synthetic vectors and cultured mammalian cells. Of course, these mammalian vectors must be compatible...Instead, reporter genes are often employed to easily monitor transfection efficiencies and expression levels... mammalian vectors...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...typically found as a single copy. Unlike the ARS vectors, CEN vectors are stable without integration. Yeast Episomal...plasmid maintenance within an E. coli cell, but vectors can be widely utilized across many different cell...core replication and resistance features of yeast vectors and explain how they differ from the bacterial ...previously described. Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex ... and yeast cells. To be useful in the lab, the vectors must contain a yeast-specific origin of replication...replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed...

  14. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...Science Mentors series: Part 1: "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring ...choose an advisor/principal investigator (PI) that will also serve as a good mentor. To read more about what...choosing a lab for postdoctoral work. However, selecting a lab with a PI who is a good mentor is important ...to learn about her mentoring style. How can I find out if an advisor is a good mentor? You have to ask ...on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s focus on how to ...what makes a good mentor, see the previous post in this series. Check out Joanne's Reddit AMA Different...Different types of mentors for grad school vs. postdoc Choosing an advisor to complete a successful PhD is different...

  15. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670...retro) Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged ...Addgene’s viral vector services allow scientists to conduct experiments faster, skipping the preparation...production, purification, and titering. Our newest viral vector service, AAV Packaged on Request  has seen a fantastic...serotype, and a dTomato under the control of the E6 regulatory element, which targets it to VIP cortical interneurons...Hub can help, with over 100 user-submitted viral vector reports. And don't forget to use the link below...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...the first base transversion editor The story of base transversion editors starts with researchers in the...Cytosine Base Editor Converts C → T; the first type of base editor invented. ABE Adenine Base Editor Converts...a glycosylase base editor (GBE). GBE Glycosylase Base Editor A base editor that relies on DNA glycosylation...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...transversion editors. Red arrows indicate a conversion directly catalyzed by the base editor. Black arrows... depends on cellular factors. Multiple labs tried to identify beneficial factors to include in even better...DNA repair proteins to the base editor, they added the pioneer factor SOX2, which is capable of binding...

  17. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV.CAGFLEX... new preps available in the Addgene viral vector repository with a nice cup of hot chocolate is absolutely...retrograde Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged ...Hub can help, with over 100 user-submitted viral vector reports. And don't forget to use the link below...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  18. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ... Lucky Thirteen: Guest Contributors of 2023 8 Great Depositor Research Stories Interested in contributing...Kits We are very grateful for each and every contributor to the blog. Thank you! More resources on the...

  19. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ... Antibody Characterization Laboratory Cancer Any Data repository From the Frederick National Library...notes) Any Data repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base...Data repository See blog post mAb3D Atlas Mouse and human brain  IHC Data repository See...there are websites that can help: Antibody data repositories, which share validation data, and antibody search...using antibody search engines and antibody data repositories to find an antibody for your experiment. Search...available antibodies from many vendors, while data repositories share validation and experimental data to help...familiar with in Table 1.  Table 1: Antibody data repositories and search engines Website Targets Application...

  20. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre... new preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged... AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab at the Allen Institute...carried out before. We have a bunch of other new AAV vectors as well — including next-generation biosensors ...multiple serotypes Looking for more viral vectors? Browse the full viral services collection or check... check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...
Showing: 21 - 40 of 867 results