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We narrowed to 35 results for: Tight TRE

Showing: 21 - 35 of 35 results

  1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...factors (which repair those breaks) makes Cas9 extremely effective as a genome editing reagent. This interplay...sides of the break, we determined that Cas9 held tightly to three of the four strands of the cleaved duplex...2014) have shown that this technique supports extremely high frequencies of genome cutting and, when donor...DNA is included in the nucleofection reaction, extremely high frequencies of HDR. The detailed protocol...

  2. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...Whatever the conjugate, the molecules must somehow be tightly linked to your antibody. This chemical coupling...PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it...

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene expression...eviction. Regulated Cre expression – placing Cre downstream of promoters that are active only in certain ...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

  4. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...transported from cells, traverse the body through the bloodstream, and eventually elicit their actions on other...drugs currently available or being developed to treat a multitude of therapeutic indices, including hypertension...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

  5. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving...expression can change as well. The more frequently and tightly bound DNA is to histones, the more compacted it...

  6. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...populations. Oftentimes, you’ll be presented with a trend of two populations with basically a smear in between..., it can depend on the type of stain whether a tighter or looser gate makes sense. Here, luckily, the ...

  8. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ... histidine residues that are present, the more tightly the protein will bind to the metal ion substrate...if you need a very pure sample. Because binding strength is proportional to the length of the tag, with...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    .... At this point, the plasmid DNA sample can be treated with RNase to remove RNA contamination and you ...nucleic acids. Containinants bind cellulose less tightly than nucleic acids, so placing the dipstick into...

  11. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...curing time. pCRISPomyces for editing in Streptomyces  Streptomyces bacteria produce a wide variety of bioactive...requires only 2-3 hours incubation. E. coli and T. citrea scarless editing plasmids Sheng Yang’s lab describes..., the system was used successfully in Tatumella citrea, another species of Enterobacteriaceae, without...created two “pCRISPomyces” systems for use in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes...Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more recombinogenic than E. coli... repression in the Gram-positive bacterium, Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis...enzyme. They identified the Cas9 orthologue from Streptococcus thermophilus, Cas9sth1 as a robust and highly...

  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...toxic or insoluble proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin...—the difference being that the latter provides tighter control of basal expression. What if I don't see...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...piggyBac™, the number of integration copies can be tightly controlled by titrating the transposase to transposon...piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no. 9, pp. 525–33, Sep. 2015...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...identically to BE3, modifying a 3-5 base window 18 bases upstream of the PAM.  David Liu’s lab generated BE3 variants... window of only a few nucleotides, so they are tightly constrained by PAM availability in the genome. ...Edits 3-5 base window surrounding -18 position upstream of the PAM Kim et al., 2017 SaBE3, BE3 PAM variants...
Showing: 21 - 35 of 35 results