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Showing: 21 - 40 of 116 results
  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel, 2023. ...to protocols. In 2013, we launched the Addgene YouTube channel to add a strong visual component to our...
  2. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ... — check out Addgene’s new Quick Tip series on YouTube! In this series, we share quick tips to help you...video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols, animations, ...
  3. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...to sit down and talk with us. Tom McFadden is a YouTuber and science educator looking for creative ways...communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special thanks...
  4. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation...video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog...
  5. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates...decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing routine...
  6. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...Coast where her first job was to wash Eppendorf tubes which were to be used for 3 months before they could...
  7. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia...
  8. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...time needed to prep for an experiment (labeling tubes never takes longer than 5 minutes, right?) As a ...   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental steps are worth...
  9. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...many different things do you simply refer to as a “tube”? We took steps towards solving this problem by...Prescribing an official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS...
  10. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...synthetic biologists to perform large scale, one-tube cloning reactions. In this blog, we’ll cover the...assemble multiple DNA fragments together in a one-tube reaction. Even though most MoClo reactions use Golden...
  11. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...prescribed order. These fragments can be pooled in one tube and ligated in as little as 30 minutes! Alternatively... per cloning reaction drops dramatically, as one tube of enzyme should be enough for at least 50 cloning...
  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase the...desired density. The volume of your flask or culture tube should be at least four times greater than your ...
  13. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable, directional cloning   The ...
  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ..., mastered the art of finding how-to videos on YouTube long before he learned (somewhat successfully) ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter, for...
  15. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Antibodies may even aggregate just from dropping the tube they are contained in (Randolph et. al., 2018). ...aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced, harvested, ...
  16. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for each set up.  Wipe down... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...
  17. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing a new DNA fragment...Watson, and Ingo H. Greger. "IVA cloning: a single-tube universal cloning system exploiting bacterial in...
  18. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...ensuring the identity of each sample from the DNA tube all the way to the viral preparation. After production...
Showing: 21 - 40 of 116 results