Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 133 results for: ado

Showing: 21 - 40 of 133 results

  3. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...to take. Decide which technique you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden... has to be optimized according to the strategy adopted, and to the specific reaction you are running. ...

  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...Activate the signaling molecule and perform the assay readout.  Of course, this is much easier said than done...the samples, antibodies, activation, and assay readout used. You’ll note that the secondary antibodies...

  5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...sequential excitations wavelengths as determined by Rosado et al: see Table 2 for more details on the effect...variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ..., calmodulin or troponin C). Their fluorescent readouts are used to monitor calcium concentrations and...sensor option if you have the setup necessary for readout.   We’ve got a live one (calcium sensors...

  7. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...happens (this time, Poisson statistics), and the readout gives an absolute value of DNA concentration. By.... PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, ...

  8. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ....1038/s41597-021-00981-0 Wright, J. F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., ...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A....

  10. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...escape a threat. Locomotion can thus be used as a readout for associated emotional phenomena like stress ...2018). Locomotion can also be used as behavioral readout to study certain forms of drug addiction. When ...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...number of bands present on the strip. This type of readout allows for nucleic acid detection almost anywhere...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...

  12. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). The FLP system works in a similar fashion...PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase of the Saccharomyces...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...approach can be incorporated into a lateral-flow readout slip that allows rapid detection.  Highlights: ...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...

  14. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...targeted cells. Depending on the cells used and the readout, this could be a major limitation. An alternative...custom CRISPR screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by ...

  17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...have the proper controls or a proper behavioral readout that allows you to interpret the results of your... high quality stereotaxic frame with a digital readout. The advantage is you can “zero” your coordinates...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...inducible gene expression Brilliant technologies adopt basic biological concepts and employ them in creative...copies of targeting sequences. Scientists have adopted and employed this concept in both imaging and inducible...observed at a later time via DNA sequencing or other readout. Cellular memory is accomplished by the use of ...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...., Greenbaum, A., Ravi, N., Wu, W.-L., Sánchez-Guardado, L., Lois, C., Mazmanian, S.K., Deverman, B.E....PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski...

  20. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...easy to source, making it easy to switch from one readout method to another or change primary antibodies ...this truly does provide a relative, not absolute, readout. Normalization Overall protein expression varies...
Showing: 21 - 40 of 133 results