We narrowed to 46 results for: astl

1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...interaction with the BirA* fused bait proteins ceases. Lastly, Split-BioID and BioID identify proteins that interact...

2. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   Published

   Sept. 27, 2017, 1:23 p.m.

   ...materials and assignments.  9) Citizen science projects Lastly, if you'd like to directly involve the public in...

3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...recent advancements in various AAV technologies have vastly improved AAVs’ potential to become ideal vectors...

4. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   Published

   Feb. 7, 2019, 2:16 p.m.

   ...base pair may not be enough to prevent re-binding. Lastly, the sequence inserted by HDR may inadvertently...

5. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ...while many more have been engineered in labs. This vastly increases the possibilities when designing screens...

6. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...can be cooled and the two phases mixed together. Lastly, the PEG solution needs to be sterilized, either...

7. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...contamination. We do not normally use antibiotics. Lastly, and perhaps obviously, it is important to have...

8. Pooled Library Amplifications

   Type

   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ...any of these options work! Selecting your media Lastly, you need to decide between growing your library...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific research, choice of a technique...

10. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...You can use this plate to start liquid cultures. Lastly, you can start small overnight liquid cultures ...

11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...mixer, they meet other Mentors and Singletons, and vastly expand their networks as they naturally find people...

12. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ...induced to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited...

13. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...similar on-targeting cutting as full-length gRNAs Lastly, the effects of guide length on off-target cuts...

14. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ...when it is perceived as being done in the dark. Lastly, data is our raw product. If we don’t take interest...

15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...HDR event, then the second HDR event can’t occur. Lastly, depending on its design, donor A may be capable...

16. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...the subject after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation....

17. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...ideally be around 5 to 20 bp away from the cut site. Lastly, if a heterozygous event is desired but only guide...

18. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ... performed on cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule...

19. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ... A to distinguish single cells from duplicates. Lastly, you learned which gates should always be included...

20. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...ablation) of a restriction site through mutagenesis vastly facilitates the subsequent process of screening...