We narrowed to 48 results for: calcium

1. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

2. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

3. Fluorescent Proteins 101: History of Fluorescent Proteins

   Type

   Blog Post

   Published

   Aug. 7, 2017, 1:58 p.m.

   ...aequorin, gives off a faint blue light when it binds calcium ions, and the second, which we now call GFP, absorbs...

4. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

   Type

   Blog Post

   Published

   Jan. 8, 2014, 3:10 p.m.

   ...GCaMP5 and GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year ...

5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

   Type

   Blog Post

   Published

   Feb. 13, 2018, 2:51 p.m.

   ...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...

6. Hot Plasmids and Viral Preps - July 2021

   Type

   Blog Post

   Published

   July 27, 2021, 1:15 p.m.

   ...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

7. Sensing Neuronal Dopamine

   Type

   Blog Post

   Published

   July 30, 2019, 12:59 p.m.

   ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

8. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   Published

   July 16, 2019, 12:59 p.m.

   .... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...

9. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   Published

   Aug. 31, 2023, 1 p.m.

   ... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...

10. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...

11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...cells that are not excitable but responsive to calcium. We’d love to expand our cancer and immunology ...

12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...

13. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

14. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ..., 1997), was designed to measure intracellular calcium and published in 1997. Since that time, numerous...

15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...

16. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...

17. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

18. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed...

19. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin...

20. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ...18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming...