... as a donor plasmid containing the transposon, along with a helper plasmid that expresses the transposase...lingering expression of reprogramming factors. Non-replicating Plasmids: Generating iPSCs with plasmid-based...plasmid-based expression requires serial transfection of 1 or 2 plasmids that express the reprogramming factors...reprogramming. Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells... generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another...the dose of plasmid the cells receive over the whole reprogramming period and the plasmid will get diluted...larger plasmids have lower rates of transfection. Minicircles: Minicircles are like mini-plasmids. They...

...SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ... mediated genome modification and gene expression. The plasmids associated with this article can be obtained...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... of the integrases is supplied on its own expression plasmid or in various combinations, while the cognate...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...

... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...H. Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts...four reprogramming factors can be delivered and expressed in multiple somatic cells via various methods....Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...transdifferentiated in vivo to pancreatic β-like-cells with the expression of pancreatic and duodenal homoeobox gene1 (PDX1...transdifferentiation is usually carried out by expressing transcription factors specific to the lineage...Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced...

...move antibiotic resistance genes between plasmids or from plasmids to the bacteria’s genome. In humans, if...Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase so they ...activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise...codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first reported active DNA...increase in transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve...for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular dystrophy...

...proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively, it equips... "Live cell imaging of phosphoinositides with expressed inositide binding protein domains." Methods 46.3...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

...PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...lines expressing it. The Church lab fixed this issue by creating a new dCas9-VPR expression plasmid that...transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila and yeast...Cas9 nickase, and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. Ordon et al., 2016 tested...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334 pCR8-Magneto2.0...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...

...as close as possible to the basal expression level, induce expression at an opportune time or go all out... following the expression pattern of a defined promoter region, ectopically expressing YGOI in a pattern...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the CMV ...for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression vectors... in molecular biology to isolate, multiply, or express the insert they carry in the target cell. These...most important thing you need to know is your expression system or environment. The host organism will...

...deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core...demethylation in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation ... are an additional layer of control over gene expression that go beyond genomic sequence. Dysregulation...modifications are correlated with alterations in gene expression, but causality and mechanisms remain unclear....that help package DNA, were associated with gene expression or silencing. To alter DNA modifications, researchers...well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds H3K27ac marks...Tet1CD, is available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure...

... pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein ...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G ...control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster, as ...

...gene is expressed in normal individuals (in a parent-of-origin-specific manner), but the expression or silencing...Epigenetics is the study of heritable changes in gene expression that do not involve any changes to DNA sequence...is wrapped around histones changes, then gene expression can change as well. The more frequently and tightly...compacted region and results in a loss of gene expression. In this way, genes in compacted regions are ...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...opens up a new way to visualize changes in gene expression and may lead to the development of a whole new...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...

...available in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls...Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 ... the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches ...the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification...