... (4). Otherwise, using a specific system for controlling gene expression such as Cre-lox, may be sufficient...and optogenetic AAV-based tools to study neural control. Surprisingly, little research has compared and...such as microRNAs (miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger...

...lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors...your gene and the marker can be placed under the control of two different promoters. Both the single and...derivative such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer...

... under the control of the cI857 temperature-sensitive repressor. The temperature-controlled expression... at a dose and duration sufficient to kill all control cells and surviving “mutant” colonies were deep...GTPases involved in cell signaling pathways that control many cellular processes such as differentiation...expressing GFP, tetracycline response elements, and shRNAs many of which are available at Addgene. Since GMAP...gRNA backbone plasmids with Addgene. Additional control plasmids for the TKO library will be available ...luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. The system...Furthermore, dCAS9-VPR expression is under the control of the TRE promoter, which allows for doxycycline-inducible...