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We narrowed to 109 results for: crispr library

Showing: 21 - 40 of 109 results

  1. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources...the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid...help you find new CRISPR tools for your research! This time: E. coli genome-wide CRISPR inhibition Covalent...editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition library To expand ...Zhang lab developed a genome-scale CRISPR inhibition pooled library (~60,000 gRNAs) that targets ~4,000...Communications Find the E. coli genome-wide CRISPR inhibition library Covalent tethering of DNA repair template...All-in-One AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform...

  2. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...10.1186/s12915-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed... damage response and DNA repair.  A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem... pneumococcal strains. The BARBEKO sgRNA library uses CRISPR cytosine base editors to disrupt gene function...A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use viral preps   Improved...the past couple months including several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins...

  3. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...Addgene Blog CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ...With the advent of CRISPR, genome-wide knockout screens are possible with gRNA libraries targeting all known...between the two, it’s time to check out our CRISPR library resources or our RNAi resources and go fishing...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest...

  4. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...yeast CRISPRa plasmids here Reduce the scale of your CRISPR screens by using this lentiviral CRISPR human...for use in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items ...Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox with their...sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...engineered prime editors here A lentiviral CRISPR knockout library that targets human epigenetic genes was...human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired guides per...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...2021.01.30.428974   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... activity to SaCas9. A new yeast inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with ... Here's what you'll find in this post: Pooled library to study antibody escape in SARS-CoV-2 Recombinant...Nanocompartments for yeast bioproduction pathways The CRISPR corner New ready-to-use viral preps Mapping antibody...Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations ...protein.   Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr et al Cell...

  6. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    Published
    Dec. 11, 2017, 2:02 p.m.
    ...new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools... out and knock down libraries. Listen to Our segment on the B. subtilis libraries. Hot Plasmid 2: Transcription...TZAP Hot Plasmid 4: CRISPR tools for use in plants.  Listen to the segment on CRISPR tools for plants. ... crispr...

  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...MORF library is most immediately useful for TF library screens. Previously engineered TF libraries were... The transcription atlas and library The transcription factor library The human genome encodes approximately... capacity. Similar to the Human TFome Library, the MORF library can be used with other selection and screening...community. Recently, the Zhang lab generated a TF library (aka the MORF Collection) and an accompanying expression...sites. The Zhang lab generated a comprehensive library of each unique TF open reading frame (ORF), barcoded...packaging and delivery to target cells as a pooled library, which will amplify each TF to determine how its...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...systems, especially lentiviral vector-based CRISPR pooled libraries that allow functional screening of thousands...Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of 96 retroviral...up-to-date information. Check our CRISPR guide and blog to learn more about CRISPR and the most recent developments...prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd generation...original lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents...by delivering shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery systems...expression, gene knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...screening diverse libraries of genetic variants. Several methods for generating mutant libraries exist, but ...oligonucleotide libraries of a discrete size at the target site. The use of such libraries means that the...Resources on Addgene.org Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...within a user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed...MAGE) will always be limited by the size of the library used. EvolvR: key components The EvolvR system... Pablo Perez-Pinera. "The continuously evolving CRISPR barcoding toolbox." Genome biology 19.1 (2018):...PMCID: PMC6154929. Halperin, Shakked O., et al. "CRISPR-guided DNA polymerases enable diversification of...

  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...screening libraries, direct edit optimization, and more. While there are many CRISPR-Cas libraries and tools...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...genetic circuits and would benefit from a larger library of parts but don’t need to add tags to your proteins...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...targetability and effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique...biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both monocots...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection ...podcast segment on CRISPR tools for plants CRISPR inhibitors Bacteria utilize CRISPR Cas9 systems to defend...Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library. Although B. subtilis...vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library targets 289 genes...on Coselection with Ouabain New CRISPR base editors The popular CRISPR base editing technique uses dCas9...knockdown libraries The Gross Lab at UCSF recently deposited 2 Bacillus subtilis libraries with Addgene...back using CRISPR inhibitors. Several such inhibitors have been characterized for type I CRISPR systems,...

  12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ... iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled...conventional CRISPR screens require a low multiplicity of infection (MOI) during cell library construction...'s lab at Peking University has developed a new CRISPR screening method that makes use of guide RNAs with...loop region. After infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be ... and their iBAR sequences. Since the sgRNAiBAR library covers every annotated human gene, it enables genome-wide...cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome Biology, 2019. PubMed PMID...

  13. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... thetargeting sequences of guides across 8 CRISPR libraries. Samples are divided into those of African...these and any other CRISPR screens using the same reference genomes and libraries. Further complicating...extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking to uncover... and Boehm labs pulled data from more than 1000 CRISPR screens in DepMap to see if they could identify...Since about 90% of the cell lines used in DepMap CRISPR screens are of either European or East Asian ancestry...it was coming through an unexpected source: the CRISPR guides, short pieces of RNA that bind to a cell...does the reference genome actually reference?  The CRISPR guides used in the screens they analyzed were designed...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...on Your CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled ...How to Conduct Genome-Wide CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org...choice. For a genome-wide library to conduct genetic screens, however, a library composed of the latter ...not quite as broad as for knockout via CRISPR cutting. For CRISPRa, it is most-efficacious to target a ~... Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to...considerations before you start an experiment with CRISPR The hammer, the jigsaw, and the wrench are all ...the same is true when designing gRNAs for using CRISPR technology – the “best” gRNA depends an awful lot...

  15. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... other CRISPR/Cas systems used for endogenous protein tagging, please visit our website CRISPR/Cas Plasmids... of interest with a fluorescent protein fusion. CRISPR genome editing can help accomplish this goal. The...sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists to tag and light up endogenous...better understand normal protein function. However, CRISPR protein tagging does have its limitations, especially...plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists...template. SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent (EGFP, ...combinations of connector and tag donor plasmids from the library can be used to generate functionally unique repair...

  16. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...reversible CRISPR modification. In addition to these subpages, our updated CRISPR Guide and CRISPR History...Heaven: CRISPR and AAV Day 6: Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials...for Pooled Libraries Day 12: New website resources: CRISPR Plasmids and Resources and CRISPR History and...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that’...popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR plasmid...tools: How to Design your gRNA for CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology... repair: CRISPR 101: Homology Directed Repair and 3 Tips to Improve HDR Efficiency for CRISPR Editing ...

  17. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...quickly create Illumina sequencing libraries. From start to finish the library preparation process only takes...the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with the...for the library in question. We will order primers that can amplify the insert of the library. This PCR... we provide, including viral preps and pooled libraries. As anyone who has ever worked to sequence DNA...for six plates (576 individual plasmids)! These libraries are then QC’d, pooled, and prepared for Illumina...Sequencing (VGS). QC is performed throughout the library preparation process and includes Picogreen quantification...electrophoretic gel, and qPCR quantification of the final libraries. A broader view of the whole plasmid To confirm...

  18. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...(2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods..., Wang X (2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome... More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on...Catch Up on Your CRISPR Background with Our CRISPR Guide Pages ... Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation for genes...Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and has taken...loci to create changes in phenotypes. For example, CRISPR activation has been used to differentiate induced...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...transfections, preserving cell viability. Library delivery Screening libraries, large collections of biological...transcription factors, barcoded libraries to trace cell lineage, and so much more! Libraries allow you to test thousands...inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...increasingly popular tools with many applications. These libraries include sgRNAs targeting the entire genome, overexpression... instead of just one at a time. Many of these libraries are packaged and delivered with viral vectors ... host cell) can be tuned so that the copies of library cargo delivered are controllable. For many screening... is essential to deliver a single copy of your library cargo to isolate the effects of each variable being...

  20. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...need to amplify a library for your own experiments. Library amplification 101 Library amplification seems...More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled Libraries at Addgene ...plasmid libraries containing hundreds, thousands, or even a million plasmids. These libraries are some...for most libraries. Here are the key points you’ll need to remember when undertaking library amplification...individual plasmid in the library. Bacterial growth must also be optimized to maintain library diversity. If the...other cells. For libraries where the plasmids are all very similar sizes, like a gRNA library, this is less... of the library should be confirmed using next-generation sequencing (NGS). For gRNA libraries, you’ll...
Showing: 21 - 40 of 109 results