We narrowed to 23 results for: dna oligonucleotides

...simultaneously incorporate multiple single-strand DNA (ssDNA) oligonucleotides (oligos), and thereby rapidly create...of recombinant DNA technology has allowed molecular biologists to fuse fragments of DNA together in a ... enables transient suppression of DNA repair during oligonucleotide integration, allowing MAGE in otherwise...sequence. Should one of these random stretches of DNA contain an enhancer, it will activate its own transcription...process alone involves much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers... platform for generating both simple and complex DNA constructs Have you ever wished you could design...Assembly method, which allows for easy assembly of DNA fragments by utilizing regions of sequence homology...

...QCascade DNA-targeting module with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using...machinery to promote homologous recombination of linear DNA fragments. Since recombineering does not contain ...blocking the elongation complex from binding the DNA or extending the transcript. Different Cas orthologues...could be further refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on the ... Then, pCRISPR is introduced along with an oligonucleotide repair template. Through recombineering, the...gRNA(s), and the repair template is supplied as a dsDNA fragment. Each round of editing takes two days, ...s41564-018-0327-z Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing...

...recognition sites that flank a DNA module in an inverse orientation, so that multiple DNA components can directionally...cloning kit simplifies the cloning process for complex DNA constructs, particular for those involving recombination...easier exchange and re-use of existing elements. DNA sequences for a desired component are cloned into... Single Strand Annealing (SSA) in the event of a DNA break. Next, the gRNA being tested is expressed along... coli and Salmonella typhimurium) by integrating DNA sequences either sequentially or simultaneously. ...nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells. (Guilinger et ... and BpiI/BbsI to efficiently assemble up to six DNA fragments at a time. This method (based on the Golden...