We narrowed to 23 results for: donor sgrna

...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses...gene are supplied to the reaction from a prebuilt donor plasmid library, available from Addgene. Site-specific...available). Second, they co-transfected short-arm donor vectors containing mAID and either Neo or Hygro ...that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against...obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA with MS2 hairpin binding sites, which then recruit...

...crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array, CRISPR-transposons...sequence which could be adjusted by changing the sgRNA length. The system could be further refined by the...

...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016... be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. ...