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We narrowed to 294 results for: expression vector

Showing: 21 - 40 of 294 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is... 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our second most ...plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9 and your gRNAs and was also ...(PX461) expresses both a Cas9 protein and a gRNA. But instead of Cas9, this plasmid expresses Cas9n (a...pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists...packaging plasmid from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be...

  2. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...applications Gene expression control Researchers are always wanting to manipulate gene expression – turn it up...interest, and often a selectable marker. This vector can express your construct in target cells transiently... of these expression control mechanisms require a specific modification of the viral vector for your gene...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ... terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical...

  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...blast LentiCas9-blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute... is a dual-vector system, meaning it is typically used in conjunction with a second vector that delivers...lentiGuide-Puro. The alternative single-vector systems typically express Cas9 and a gRNA from the same plasmid...constructs (i.e., using a dual-vector system as opposed to a single-vector system) has several benefits....stable Cas9-expressing cell line and then transduce this line with lentiviruses expressing various gRNAs...transductions with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic...and transgene expression across different cell types (Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry...

  4. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...Production at Addgene Viral Vectors 101: What is a Virus? Viral Vectors 101: Viral Vector Elements ...and viral particles are packaged, when the vector is expressed. Once the design and construction steps are...integrated into the host genome? Expressed episomally long-term? Expressed short-term? Answering these questions...nucleic acid cargo of your choice. First, a viral vector is selected to perform the transduction with: either...desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic acid will be... it’s time to make virus! The constructed viral vector containing the cargo of interest is then typically...293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

  5. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ... and other viral vector tools in the years to come. We’ll make many more viral vector tools and resources... A Versatile Tool for Gene Expression in Mammals Subscribe to Viral Vector Blog Posts Additional Resources... Service Page Browse Additional Viral Vectors Find Viral Vector Protocols ... retrograde (the most popular form), this viral vector is used to deliver DNA encoding Cre recombinase...synapsin promoter. It can be used to drive Cre expression specifically in neurons. AAV Delivered Cre - ...further control where your gene of interest is expressed.  A cell-type specific Cre can provide you with...Cre-dependent mCherry line with a separate line that expresses Cre in neurons, but both of these options would...

  6. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...Viral Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting Viral Vectors 101...fact, of how adenoviral vectors work. What are adenoviruses?   Adenoviral vectors are commonly derived from...majority of adenoviral vector systems used in research, they are only expressed in the producer cells ...cells that are infected by the vector, only the transgene will be expressed, since the early genes are absent...encapsidate new virions) are not expressed. Note that replication-competent AdV vectors also exist and are used...adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases...retroviruses). The tradeoff is that adenoviral vector expression is very short-lived. It is worth noting that...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications,...and future) applications of lentiviral vectors! Lentiviral vector history and components In the early ...viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The vectors could...transgene expression, but they could only infect actively dividing cells. Another type of vector, based ... but without sustained transgene expression. To design a viral vector system that could do both, Addgene...showed that expression persisted for at least thirty days. Although adeno-associated viral vectors can also...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...know what expression pattern you need, and the factors in your system that can affect expression, before...early gene expression, but can become methylated over time, resulting in decreased expression (Damdindorj...tyrosine-hydroxylase (TH), can limit which cells express your gene. Off-target expression can still occur (Fig. 3) - for...transiently express TH - so you’ll need to check for this in your system and then test each vector before ...timing of expression in your experiment. Some inducible constructs can exhibit leaky expression in the absence...one that optimizes gene expression where you want it and minimizes expression where you don’t. The key...on the Addgene blog Viral Vectors 101: An Introduction to AAV Viral Vectors 101: The AAV Retrograde Serotype...

  9. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Knockdown the expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression Level of Gene... the empty vector control shows any effect of the vector/backbone itself on gene expression in your target...as the expression level of Gene X in cells treated with Plasmid A is similar to the expression level of...empty control vector would be Plasmid A sans shRNA, or backbone Y alone. The empty vector control allows...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X....replicate controls.  Transfection controls: empty vector and internal control A transfection control measures...any effects of the transfection itself (i.e., the vector used in transfection, transfection reagent, or ...

  10. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Pseudotyping Viral Vectors 101: Systemic...transgene expression and transduction efficiency (Shitik et al. 2023). Transgene expression can be altered...ITRs can be used to drive gene expression at varying capacities. AAV vectors containing the AAV2 ITRs can...Inverted Terminal Repeat Sequence Role in Vector Transgene Expression. Hum Gene Ther, 31(3–4), 151–162. https...important biological tool that allow scientists to express a gene of interest in a cell or animal model. AAV...and are required for replication, packaging, and vector persistence. They work in conjunction with the ...retained with the gene in a recombinant AAV (rAAV) vector (Figure 1). Everything else outside the ITRs gets...

  11. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... existing transposon and vector systems and creating an all-synthetic vector that included only the elements... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum... to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke et al.,...step, compatible Level 0 vectors are directionally assembled into a Level 1 vector creating a single transcriptional...Cre-based Brainbow-expressing cells. Autobow plasmids are all-in-one versions which express self-excising ... May 5;10(6):540-7. pCoofy vectors for optimizing protein expression Under the direction of Sabine ...

  12. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...targeting receptor expression as a ligand and receptor pair. Targeting Receptor Expression Using chemogenetics...therapies when expression must be restricted to specific circuits. Methods for targeting expression involve ...on the Addgene blog New to viral vectors? Browse our Viral Vectors 101 blog posts Read blog posts about...target effects. For example, if your receptors are expressed in the brain, it is necessary to use ligands capable...different responses depending on what cell type it is expressed in. The most common target for chemogenetic manipulation...modify other properties such as endogenous gene expression, which can lead to changes in normal cell physiology... H, Fishman GI, Conklin BR (1999) Conditional expression and signaling of a specifically designed Gi-coupled...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...blue are the predominate colors expressed (figure 4c).   AAV Vector  Percent of Neurons Transduced   ... color expression into a two part system (figure 4d):  Part 1: A cocktail of three AAV vectors expressing...using AAV as a tool for gene expression in mammals. Subscribe to Viral Vector Blog Posts Additional Resources...   Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study...are easy to manipulate, 2) they have long-term expression; and 3) they have limited toxicity. However, ... technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated. Diversity is introduced...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...AAV vectors. I wanted to use an AAV vector to express ChR2(H134R) in A2 norepinephrine expressing neurons...serotypes containing an expression cassette with an ubiquitous promoter to express GFP in a group of young...to AAV vectors, many from people trying to answer the exact same question I was: What AAV vector is best...evaluate how small changes in vector administration can influence AAV expression properties.  By providing...Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists... brain region or nucleus. Just optimizing AAV expression can take months (or longer), yet the process ...question and used it to publish!  Working with AAV vectors in neuroscience Let’s consider my first challenge...

  15. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...ubiquitous expression. pAAV-mDlx-NLS-mRuby2 (99130-AAVrg): The Dlx element directs expression in forebrain...directs strong, ubiquitous expression. This is a Cre-dependent switch that expresses nuclear mCherry in the...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...pAAV-EF1a-Flpo (55637-AAV1) Viral vectors coming soon! These vectors should be packaged and available ... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the...entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors and ...

  16. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... of vectors for the SB transposase system - one set of vectors for robust constitutive expression (pSBbi...gene (red) for expressing the transposase enzyme.2.) The transposase enzyme is expressed (red stars) and...efficiencies of integrating viral vectors. New Sleeping Beauty transposase vectors Even more recently, Eric ...pSBbi derivatives) and one set of vectors for inducible expression (pSBtet derivatives). The constitutive...constitutive and inducible expression vectors were developed into 16 variants with different selection markers...cells’ defense mechanisms. The use of non-viral vectors, like transposons, avoids many, though not all,...transposition; and 2) a transposon containing the gene-expression cassette that can translocate within the genome...

  17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...transfected with both vectors. Flex vectors are the solution. Flex vectors expressing fluorescent cytoplasmic...concentrations of vectors expressing the required recombinase. The high concentration of FLEx vectors guarantees.... It became possible to design FLEx vectors (Flip-excision vectors) that only allow one inversion event...types in neuronal networks Double inverted vectors expressing light inducible ion channels (plasmid # 20298...cell specific manipulations in vivo, FLEx vectors expressing the light activatable ion channel under the...specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow...means to spatiotemporally control FLP and Cre expression have been developed. Read on to learn more about...

  18. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... alters or eliminates the expression of a particular gene. Targeting vectors used to create knockout mice...Inducible gene expression Another type of genetically engineered mouse model allows gene expression to be controlled... that regulates Cre expression. The most common drug used to induce the expression of Cre is tamoxifen...of the Esr1 domain that blocks Cre expression. Then, Cre is expressed and Cre-mediated recombination of...Mice can be engineered by inserting a targeting vector containing the gene of interest into a zygote, ..., and mice with conditional or inducible gene expression. Transgenic and knockin mice As the name suggests...microinjection of DNA or infection with a viral vector containing the gene construct can be used to insert...

  19. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... have found that expression occurs quickly and results in long and stable expression (up to 9 months in...the onset of gene expression. However, it has yet to be determined what gene expression looks like at 3 ...-associated viral vectors: packaging capacity and the role of rep proteins in vector purity." Human gene...Tool for Gene Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional...Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting my graduate program at the...in locomotion. We have relied heavily upon AAV vectors to deliver chemogenetic/optogenetic constructs ...Although they have a small packaging capacity, AAV vectors were suitable for our needs for the following reasons...

  20. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ... an episomal vector system that increases reprogramming efficiency through the expression of a shRNA against...RNA virus Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming factors...Reprogramming efficiency of PBMCs with episomal vectors Episomal vectors are based on the Epstein-Barr Nuclear ...long-term expression of the reprogramming factors. However, reprogramming of PBMCs by episomal vectors has ...human cells, thus expressing additional EBNA-1 transiently. The transiently expressed EBNA-1 increases ...protein expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector system...common goal of all reprogramming methods is the expression of the reprogramming factors- OCT3/4, SOX2, KLF4...
Showing: 21 - 40 of 294 results