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We narrowed to 39 results for: feng zhang

Showing: 21 - 39 of 39 results

  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., ...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9...of a sunny day in Edinburgh.   References 1. Gao, Feng, et al. "DNA-guided genome editing using the Natronobacterium...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al...end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13...Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA editing toolkit to allow C ...

  5. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex ...Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease...

  7. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...) Cas13a targeting with a single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a...Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (...

  11. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries ...different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6),...

  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from....0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids...

  15. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...PMC2629495. Zingg, B., Chou, X., Zhang, Z., Mesik, L., Liang, F., Tao, H.W., and Zhang, L.I. (2017). AAV-mediated... S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K.E., Bohn,...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Cummins, A., Chen, Y., Fan, Y., Zhang, Q., Sharma, J., Duan, Y., Wang, L., Feng, G., Chen, Y., Ip, N. Y., Pickel....2022.05.003 Chen, X., Wolfe, D. A., Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan...Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G. A., Wade, K., He, J., ...Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., ...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...domain and a mostly c-terminal nuclease domain. Feng Zhang's group utilized the bi-lobed architecture of ... of varying fusion protein expression levels. Zhang et al., PLoS One. 2012;7(8):e43072. doi: 10.1371...
Showing: 21 - 39 of 39 results