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We narrowed to 42 results for: fp

Showing: 21 - 40 of 42 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... to an FP; when supplied with a luciferase substrate, the luciferase transfers energy to the FP, resulting...limited by a few caveats. Since fluorescent proteins (FP) require external light activation, you can’t use...you use the excitation wavelength to specify which FP emission you’ll capture. Since the Nano-lantern system...

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently...recently developed a series of blue, green, and red FPs that are codon optimized specifically for expression... on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized...

  3. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...a synthetic intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.... homology arms for your gene of interest into the FP-SEC vector using Gibson assembly. This construct ...creating a loss-of-function allele as well as a promoter-FP fusion for the gene of interest. L1/L2 larvae from...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...proteins (FPs) are the gold standard for genetically-encoded FRET donors (Fig. 1). Scores of FP variants...experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa of protein... due to this bulky insertion are inevitable. Most FP fusions for FRET studies are at the N- or C-terminus...function of certain proteins. Rather than shove a bulky FP into your protein, you can site-specifically label...

  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin ...protein interest would also result in depletion of the FP, which can be easily visualized.  The tool-kit consists...CRISPR techniques. This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG...

  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...same time in live cells using fluorescent proteins (FPs) can be problematic because excitation light can ... biological phenomena. The technique of coupling FPs with Luciferases can overcome these problems but ...engineered Renilla luciferase, to produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these...coelenterazine intiates the fluorescent signal and these FP-RLuc8 fusions were dubbed Nano-Lanterns. RLuc8 is...

  7. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...you! In almost any case you would use a traditional FP fusion, a SunTag fusion could be used as well. Pros...kDa vs 24 kDa for GFP alone. Although traditional FP fusions can also affect protein activity, half-life...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...pushes NLS-PCP-GFP off of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear...protein binding sites to tether many copies of the FP-coat protein fusions to their reporter mRNAs. Using...

  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...calcium reporters use a single fluorescent protein (FP), requiring only a simple measurement. However, single...biosensor. Ratiometric measurements with certain single FPs (such as GEX or blue/green CEPIA) or through FRET...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...RFP variants The toolbox of fluorescent proteins (FP) for cellular imaging is constantly expanding. Case...the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert Campbell’s lab... to learn about the influence of structure on an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift...various RFPs. For tips on how to choose the right FP for your next experiment, check out our recent guest...

  16. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ... protein combination, for example, with a far-red FP, would increase the available colors to 15! They ...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...protein binding sites to tether many copies of the FP-coat protein fusions to their reporter mRNAs. Using...-targeted photoactivatable fluorescent proteins (FPs). A subpopulation of mitochondria is selectively ...selectively labeled by irradiating the photoactivatable FP, then the overlap between labeled and unlabeled mitochondria...mitochondrially targeted photoactivatable and photoswitchable FPs that are activated by UV light (300-400nm) and that...

  19. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...HeLa cells were transfected with plasmids encoding FPs tagged with the plasma membrane targetin sequence...
Showing: 21 - 40 of 42 results