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We narrowed to 60 results for: fret

Showing: 21 - 40 of 60 results

  1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...considered a linker (figure 1). In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai...fluorescent protein can be truncated to increase the FRET efficiency. Creating fusions at the N- and C- termini...Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness,...PubMed PMID: 30886412. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in.... van Unen, Jakobus, et al. "A New generation of FRET sensors for robust measurement of Gαi1, Gαi2 and...

  2. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities: Tracking intracellular...Central PMCID: PMC3984138. Microscopy U. Basics of FRET Microscopy. Additional Resources on the Addgene...Fluorescent Protein Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute...Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent Protein Guide ...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...cause an effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy...excites the fluorescence of another FP (e.g. YFP). FRET only occurs when the distance between the two FPs...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins interact...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been... of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions between...concentrations and pH, using a range of strategies such as FRET, calmodulin, and others. Review Addgene's collection...

  5. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... tips from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers...Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays ...Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling...

  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...Filonov et al., 2013). FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1...indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were...optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A...

  7. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation complementation...used to study binary protein interactions. However, FRET generally only tells you if two proteins interact...Find Plasmids from the Roux Lab Find Plasmids for FRET ...

  8. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...     Additional Resources Learn how you can use FRET to study protein interactions Learn about other ...CRISPR plasmids for isolating a genomic locus Browse FRET plasmids for studying protein interactions  ...

  9. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...information, accession numbers, sequences and lineages, FRET efficiencies, additional references, etc...) and...all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein...

  10. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...This technique is very similar to the widely used FRET, but it eliminates the need for an excitation light...Check Out All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein...

  11. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...luminescent) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy from an excited donor...Luciferase Fluorescent Proteins 101: Introduction to FRET Resources on Addgene.org Luciferase collection...

  13. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...using fluorescently tagged ligands or intermolecular FRET for ligand-receptor interactions, require specific...tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to have compatible ...profiles is vital for the accurate interpretation of FRET-based experiments. A popular combination is Alexa594...

  14. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Smith et al., 2024). They then used single-molecule FRET to study the intramolecular dynamics of the proteins...2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a...

  16. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...molecular biology methods such as allelic exchange, FRET, and synthetic biology. 7. 25 Work From Home Tips...

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM...Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the...mammalian mCyRFP1 or CyRFP1 expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv,...

  18. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...foundation of multiple important technologies, including FRET and optogenetics. Even though GFP has been so thoroughly...

  20. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...also investigate protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF ...
Showing: 21 - 40 of 60 results