We narrowed to 58 results for: gag pol

... directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski then used... a duplicated SV40 polyadenylation region instead of the synthetic polyadenylation signal present in pGL3...system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the light-inducible protein... accessible for many potential applications. Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3)...recruits up to 24 copies of a protein to a target polypeptide. Multimerization in the SunTag system occurs ... simplifies the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series...constitutive promoters, 2 includible promoters, 3 polyA terminators and various pieces for inducible expression...

...revealed that young scientists are aware of, and are engaged in, these issues which will continue to shape the.... The topic of unionization continues to be a polarizing one. High-profile universities remain outspoken...science funding and funding mechanisms require an engaged sector with effective advocacy skills. Advocating... panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office Ben Corb - Director... in the intersection between science and public policy. Follow David on Twitter @driglar.   References...

...discussions with both panelists and audience fully engaged. They can also be dull and painful to sit through...well-planned approach to making the event interesting and engaging. Long before the event... Choose a moderator ...advantage of the program. Start the program by polling the audience with a few questions. Who is out there...in response. I am always ready with some written poll questions to get things started. Give a couple of...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect...

...directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski used SITS...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He has also constructed a library ...systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set...

...meetings. When scientists tell good stories, they engage their readers or listeners thus expanding the impact...it for a while and he eventually calmed down, apologised and admitted he’d been under a lot of pressure...invite people to ask questions, be approachable. Engage with your audience and try to learn from them. ... again until you work out what people enjoy and engage with and what parts you can leave out. Start viewing...

...presenter, it can be difficult to keep the audience engaged through a computer screen. For the Reproducibility...organizers found several avenues to keep the audience engaged. They first sent a pre-workshop questionnaire to...are still other ways to make your session more engaging, for example, by using short animations and visuals...visuals, or asking questions using the polling feature or the chat box. One key thing to remember regardless...

...are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I’m trying...classroom are characterized by intense student engagement and joy, rather than a perfect lesson plan or... this fall:  The BioBuilderClub BioBuilderClub engages high school teams around the world who combine ...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

...curious feature of this Cas is that once it is engaged by its target, it will non-specifically cut ssDNA...indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why would you want to give...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target sequence. The RNA-targeting ability...

... development, business development, consulting, policy, big data and the list goes on and on. As you think...Hire American" Executive Order which sets broad policy intentions directing federal agencies to propose...your company employs at least 3 full-time people engaged in researcher activities. The EB-1B requires that...meets the requirements set forth by USCIS before engaging with clients. To learn more, visit www.casseuslaw.com...

...so—can prove invaluable in the pursuit of public engagement. Studying science communication is an equally...had to say. Moreover, this subject allowed me to engage in findings relevant to my background (neuroimaging...sport athletes, as well as the ethical and health policy implications arising from sport-related concussion...

...competitive skill sets in a myriad of professions is to engage in extracurricular activities (EA). EAs can: Introduce...expands your professional network. No brainer! Engaging audiences from across the hall, fields, and other...editing manuscripts? Are you interested in scientific policy? Next, use the resources below to identify potential...

...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...

...biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...dissonance (Kunda, 1990). More specifically, they were engaging in a behaviour called the “biased evaluation of...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...

...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are...Na+) against the concentration gradient and hyperpolarize the cell (Mattis et al., 2012). Bicistronic ...the receptor, when exposed to light, the ligand engages the binding pocket and activates the receptor. ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they... A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES is an optogenetic tool developed for bidirectional...

...amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...message delivered by many of the speakers.  As Dr. Polka said as she started things off, “We are all capable... energy and to choose institutions with allies engaged in training excellence to begin effecting change...

... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

...not just wait for papers to be published, but to engage in ongoing, real-time dissemination of information...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

...plasmid? Figure 2: Survey results from a Twitter poll run in 2023, n=194.   After the great Plasmid...much harder to calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

...to express the transcription factors from a polycistronic cassette. However, it was unknown whether variations...variations in gene expression from these polycistronic cassettes could lead to comparable experimental...Woltjen Lab compared different variants of the polycistronic cassettes and discovered reprogramming discrepancies...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...reprogramming and global gene expression patterns. Polycistronic cassettes that contained the KLF4L isoform had...the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work...activity than BE3  85172  pBK-EQR-BE3  EQR-Cas9 (NGAG)  Lower off-target activity than BE3  85173  pBK-VRER-BE3...

.... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...