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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 58 results for: gag pol

Showing: 21 - 40 of 58 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski then used... a duplicated SV40 polyadenylation region instead of the synthetic polyadenylation signal present in pGL3...system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the light-inducible protein... accessible for many potential applications. Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3)...recruits up to 24 copies of a protein to a target polypeptide. Multimerization in the SunTag system occurs ... simplifies the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series...constitutive promoters, 2 includible promoters, 3 polyA terminators and various pieces for inducible expression...

  2. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...revealed that young scientists are aware of, and are engaged in, these issues which will continue to shape the.... The topic of unionization continues to be a polarizing one. High-profile universities remain outspoken...science funding and funding mechanisms require an engaged sector with effective advocacy skills. Advocating... panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office Ben Corb - Director... in the intersection between science and public policy. Follow David on Twitter @driglar.   References...

  3. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...discussions with both panelists and audience fully engaged. They can also be dull and painful to sit through...well-planned approach to making the event interesting and engaging. Long before the event... Choose a moderator ...advantage of the program. Start the program by polling the audience with a few questions. Who is out there...in response. I am always ready with some written poll questions to get things started. Give a couple of...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski used SITS...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He has also constructed a library ...systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set...

  5. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...meetings. When scientists tell good stories, they engage their readers or listeners thus expanding the impact...it for a while and he eventually calmed down, apologised and admitted he’d been under a lot of pressure...invite people to ask questions, be approachable. Engage with your audience and try to learn from them. ... again until you work out what people enjoy and engage with and what parts you can leave out. Start viewing...

  6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...presenter, it can be difficult to keep the audience engaged through a computer screen. For the Reproducibility...organizers found several avenues to keep the audience engaged. They first sent a pre-workshop questionnaire to...are still other ways to make your session more engaging, for example, by using short animations and visuals...visuals, or asking questions using the polling feature or the chat box. One key thing to remember regardless...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I’m trying...classroom are characterized by intense student engagement and joy, rather than a perfect lesson plan or... this fall:  The BioBuilderClub BioBuilderClub engages high school teams around the world who combine ...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...curious feature of this Cas is that once it is engaged by its target, it will non-specifically cut ssDNA...indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why would you want to give...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target sequence. The RNA-targeting ability...

  9. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... development, business development, consulting, policy, big data and the list goes on and on. As you think...Hire American" Executive Order which sets broad policy intentions directing federal agencies to propose...your company employs at least 3 full-time people engaged in researcher activities. The EB-1B requires that...meets the requirements set forth by USCIS before engaging with clients. To learn more, visit www.casseuslaw.com...

  10. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...so—can prove invaluable in the pursuit of public engagement. Studying science communication is an equally...had to say. Moreover, this subject allowed me to engage in findings relevant to my background (neuroimaging...sport athletes, as well as the ethical and health policy implications arising from sport-related concussion...

  11. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...competitive skill sets in a myriad of professions is to engage in extracurricular activities (EA). EAs can: Introduce...expands your professional network. No brainer! Engaging audiences from across the hall, fields, and other...editing manuscripts? Are you interested in scientific policy? Next, use the resources below to identify potential...

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...

  13. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...dissonance (Kunda, 1990). More specifically, they were engaging in a behaviour called the “biased evaluation of...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...

  14. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are...Na+) against the concentration gradient and hyperpolarize the cell (Mattis et al., 2012). Bicistronic ...the receptor, when exposed to light, the ligand engages the binding pocket and activates the receptor. ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they... A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES is an optogenetic tool developed for bidirectional...

  15. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...message delivered by many of the speakers.  As Dr. Polka said as she started things off, “We are all capable... energy and to choose institutions with allies engaged in training excellence to begin effecting change...

  16. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

  17. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...not just wait for papers to be published, but to engage in ongoing, real-time dissemination of information...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

  18. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...plasmid? Figure 2: Survey results from a Twitter poll run in 2023, n=194.   After the great Plasmid...much harder to calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...to express the transcription factors from a polycistronic cassette. However, it was unknown whether variations...variations in gene expression from these polycistronic cassettes could lead to comparable experimental...Woltjen Lab compared different variants of the polycistronic cassettes and discovered reprogramming discrepancies...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...reprogramming and global gene expression patterns. Polycistronic cassettes that contained the KLF4L isoform had...the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work...activity than BE3  85172  pBK-EQR-BE3  EQR-Cas9 (NGAG)  Lower off-target activity than BE3  85173  pBK-VRER-BE3...

  20. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    .... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...
Showing: 21 - 40 of 58 results