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We narrowed to 24 results for: gag pol vector

Showing: 21 - 24 of 24 results
  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...continues to be challenging. Organism specific viral vector systems would also be beneficial for many systems...not just wait for papers to be published, but to engage in ongoing, real-time dissemination of information...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...
  2. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...on the Addgene Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian...sharing cells: the black market of cell biology. Engaging in this practice would seem to save time and money...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...
  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...experiment, select from a variety of empty sgRNA vectors and design your sgRNA targeting sequence using ... Fig. 2: Prime editor engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand...
  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...sequenced and quality controlled plasmids and viral vectors. When working with a plasmid from Addgene, you ...Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility... posts on the defunct genome editing technology NgAgo). In addition, there are organizations like ReFigure.... Down the line we may see further adoption of policies that promote reproducible publishing practices...
Showing: 21 - 24 of 24 results