...institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal agreement. Below... want to wait? Talk with your TTO Has a pending legal agreement ever delayed your access to necessary ...Transfer Office. Some synonymous terms include: legal office, office of sponsored projects/research, office...

...This post was contributed by Adriana Galvan, an associate professor at Emory University School of Medicine...express hM4Di in neurons of rhesus monkeys or mice (Galvan et al., 2019). The hM4Di receptor is an engineered....     Thank you to our guest blogger! Adriana Galvan is an associate professor at Emory University School...2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie...

...Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals (@MicrobiGals) December 23, 2020   In second place,...Winning first place in the home edition, the MicrobiGals created glow in the dark petri dish ornaments...

...in Drosophila (Fig. 1):  GAL4-UAS, a transcription factor binding site. GAL4 expressed in the fly binds...of SPARC Figure 1: SPARC and SPARC2 use the GAL4-UAS system along with precisely truncated attP sites...

...References Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E...Hanekom W, Moosa M-YS, Lessells RJ, de Oliveira T, Sigal A (2021) Escape of SARS-CoV-2 501Y.V2 from neutralization...Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB...

...experimental model organism. I then described the Gal4/UAS system used by geneticists to study gene function...favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the available reagents do not match... or by filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to...

...interest. These transcription factors consist of the GAL4 or ZIF268 DNA binding domains fused to either the...fluorescent reporters and other proteins controlled by GAL4 and ZIF268. To demonstrate independent control of... Aranda-Díaz et al transformed yeast with both a GAL4/estradiol controlled transcription factor and a ...

...genes from the pYES2.1 plasmid is controlled by the GAL1 promoter. The cloning procedure adds both V5 and... to grow in the absence of methionine. Since the GAL1 promoter controls MET/Met gene expression, complementation...complementation should only occur when galactose replaces glucose as the carbon source. Student results have...

...Epitope Mass (kDa) Function Notes CBP KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification Binding and...eukaryotes TAP GRRIPGLINP WKRRWKKNFI AVSAANRFKK ISSSGALDYD IPTTASENLY FQGEFGLAQH DEAVDNKFNK EQQNAFYEIL HLPNLNEEQR...PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG  12 Solubililty...

...transducing cells: galactose and the AAV receptor (AAVR). Cell lines lacking galactose were still transduced...PHP.B, unlike its parental AAV9, does not require galactose and AAVR to transduce cells. The Wilson Lab generated...

...lead to covalent binding of the TMP-tag ligand (Gallagher et al., 2009). HaloTag: Scientists at Promega ...https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish...better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich...

... before.  These attacks on science ultimately galvanized scientists to rally in Ottawa to protest the ...AL, USA 33.5207°N 86.8025°W - Jonathan Aguilar Galileo wrote, “now you would not hide behind these two... large one” (http://oll.libertyfund.org/titles/galilei-dialogues-concerning-two-new-sciences). This is...

...está disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos...materiales de nuestro repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles...

...point where the temperature and humidity favors fungal growth. The ants bites down and locks their jaws...its muscles atrophy. After the ant’s death, the fungal fruiting bodies being to sprout out of the ant’...

...small subset of AAV plasmids will have technical or legal restrictions that make them ineligible for use in...submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility with our production...

...commonly introduced in zebrafish through the UAS/Gal4 system for behavioral studies. In neuroscience, ...different subsets of spinal cord neurons using the Gal4/UAS system. This created fish lines with different...

...as a drug-screening platform to identify new antifungals that target calcineurin. Current calcineurin ...calcineurin gene reporters are based on the bacterial βgal assay, and are not suited to high throughput screening...

... – a career in the sciences is more like an intergalactic network. Which planet will you visit? Where ...into your backpack, and step out there into the galaxy. TL;DR: Many roads need scientists open for adventure...

...Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for this project with ...out what we can afford to do and when. Addgene’s Legal Team acquired permission from relevant plasmid depositors...

...museum "Fábrica Centro Ciência Viva" in Aveiro, Portugal. If cancer was a musician, then metastasize is...museum "Fábrica Centro Ciência Viva" in Aveiro, Portugal. She is particularly interested in science journalism...