Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 40 results for: golden gate

Showing: 21 - 40 of 40 results

  1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ... to the standard restriction/ligation, Gateway, or Golden Gate cloning methods. Whether it is for routine...actually work by significantly different methods. Golden Gate cloning does result in the seamless joining of...location and NOT in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional...

  3. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...tool designs overhang sets for high complexity Golden Gate Reactions Cloning ligation reaction calculators... For general primer design Design primers for Goldengate cloning   Design primers for In-Fusion Cloning...

  4. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...restriction enzymes that are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this...our SnapGene powered plasmid maps far easier to navigate. These practical and effective changes will give...

  5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read...and the subsequent product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt...maintained. Recombination efficiency is high enough to negate the need for selection usually required in recombineering...

  6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ..., the use of another cloning method, such as Golden Gate assembly, may also be beneficial. SLIC is most...through transformation, so the cloned vector must be ligated. But in LIC, longer single-stranded overhangs can...amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility allows it...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...consists of modular parts that are assembled with Golden Gate Assembly. They built this toolkit upon the Yeast...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...

  11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden Gate Cloning Read all of our Plasmid Cloning Posts...recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated empty vectors, ...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others...focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what ...expressed the ccdB gene and were therefore unable to propagate because CcdA wasn’t available to counteract the... to thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...test different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in ...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...

  13. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... Method Technology Pros Cons References Golden Gate Type II restriction enzymes Can assemble up to...

  14. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or SLIC Cloning Read Other Plasmids 101 Posts...as this will be useful for the ligation step. 3. Ligate Your Insert into Your Vector In the ligation step...

  16. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi..., gene knockout strategies cannot be used to investigate essential genes. Historically, gene silencing... which allows easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in ...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...engineering. These components are provided in Golden Gate compatible backbones that can be combined with...all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system...system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists...neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit...empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your...toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the...2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of...

  19. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...a microscopist, a biostatistician, and that one golden-handed technician who can do a certain difficult...someone to coordinate, someone to decide, someone to delegate, etc. If you find that there is not someone (or...

  20. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... John W. Campbell. This period is termed as the golden age of SciFi, but the common themes that these ...between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing...
Showing: 21 - 40 of 40 results