We narrowed to 41 results for: golden gate

Using plasmids for transformation, cloning, or transfection? Different uses mean you'll need different purities of DNA for your applications.

June 16, 2020, 1:25 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

The PUF Assembly Kit from Huimin Zhao's lab makes it possible to devise designer PUF proteins to hit any RNA target of interest.

June 17, 2014, 7:57 p.m.

An inside look at the ten most frequently distributed plasmid technologies at Addgene from the past ten years.

Jan. 8, 2014, 3:10 p.m.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

Nov. 19, 2025, 2:15 p.m.

A list of free online molecular biology tools for plasmid mapping, DNA and protein sequence analysis, primer design and more.

Sept. 12, 2023, 1:15 p.m.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Feb. 10, 2016, 3:30 p.m.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Sept. 24, 2019, 12:55 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

June 29, 2017, 1:06 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

June 27, 2019, 12:25 p.m.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

May 9, 2017, 2:12 p.m.

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Dec. 8, 2016, 3:30 p.m.

Transient plant leaf transformation can save you time... months in fact. Learn more about this method and get some tips and tricks for designing your transient plant leaf transformation experiment.

July 25, 2019, 12:03 p.m.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Feb. 7, 2017, 3:30 p.m.

Discover the tools you need to perform plant genome engineering with CRISPR.

Oct. 11, 2016, 2:30 p.m.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Feb. 18, 2016, 3:42 p.m.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Oct. 27, 2020, 1:15 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon