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We narrowed to 256 results for: human dat

Showing: 21 - 40 of 256 results

  1. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...recently published data demonstrating that CasX is active in both E. coli and human cells. Using the structure...universal sgRNA is not predicted to target sites in the human, pig, or zebrafish genome, this system can be used..., a small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered...plasmids for active and inactive CasX for E. coli and human expression. Read the paper in Nature Find the... and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays in ...library targeting 26 genes chosen from an analysis of human metastasis cohorts and a prior mouse metastasis ...

  2. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...blog has not been updated in response to this paper. Any hint of CRISPR editing in human embryos has been...al. "Correction of a pathogenic gene mutation in human embryos." Nature. Epub 02 August 2017. doi:10.1038...

  3. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ... Catalog on our data portal. The plasmids should be functional in many different human cell types, and...the genome is still inefficient, particularly in human cell lines. Furthermore, the quality control necessary...created an initial set of 10 plasmids representing 10 human genes and will add more to the collection later .... "Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization." bioRxiv...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...especially true in the case of protein tagging to elucidate cellular structures. Overexpression methods flood...

  4. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...activation helper protein. SAM can be combined with a human genome-wide library to activate all 23,430 known...sgRNAs for each transcription start site in 15,977 human genes. The transcriptional repressor (CRISPRi) library...for each transcription start site in those 15,977 human genes. Weissman and his colleagues demonstrated ...cell types and in live mice. Therapeutic uses in humans are also on the table. “In the future, there is...we can’t rely on genetic engineering to optimize humans for CRISPRi and CRISPRa.” Before considering the...getting optimized guide sequences for activating any human gene, go to http://sam.genome-engineering.org/. ...consistency and validation with >80% effectively enriched guides per gene hit, and 100% validation of the top...

  5. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...question has been thoroughly answered by experts in human-computer interaction (HCI), and we have adhered ...common features? We plan to continue updating the feature database. Powering the plasmid maps on Addgene.org.... Development of this tool required creating a database of common features, and devising rules for identifying...when the match is imperfect. SnapGene's feature database The source of common features was our collection...issue is addressed by adding more variants to the database. Another limitation is that by tolerating mismatches.... To prevent misidentification, we augment the database with new fluorescent protein variants. The future... which common features need to be added to the database – we will work to fill any common annotation gaps...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... both human and fly versions. Arnold, et al. Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral...   Human kinase domain constructs for automated bacterial expression The misregulation of human kinases...Lab recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs...gRNAs targeting a total of 20,121 genes within the human genome (the identity of the genes and target sequences...repository that can be used to perform screens in human cells. General information regarding how to use ...receptors (GPCRs) yet one third of the GPCRs in the human genome are orphan receptors meaning their ligands...allows researchers to interrogate the druggable human GPCR-ome using a quick, affordable, and ubiquitous...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with ... wide range of human cells.  A new pooled library from Junjie Chen's lab targets human genes known or ...XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells, a cell type that has been...robust expression of high G+C content genes, as validated through monitoring the expression of fluorescent...compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered and ...genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...Addgene The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library that targets human glycosyltransferase...complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections and physiology...bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers advantages.... The exon-deletion hybrid gRNA library deletes human alternative cassette exons and disrupts genes with...new viral preps from recent months: The Dolcetto human CRISPR inhibition pooled library is now available...library format! The library inhibits over 18,000 human genes. Find the lentiviral preps at Addgene hSyn1...in length to tag the target protein. The team validated this tool by targeting six different genes whose...

  9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... purpose of making sequence-specific changes in human induced pluripotent stem cells (iPSCs). The CRISPR...mutations After genome editing with CRISPR/Cas9 in human cells was described in 2013 (Cong et al, Mai et ...PMC3795411. 2. Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (...allelic mutation incorporation probabilities our data revealed, and found that for generating a homozygous...Plasmids Check Out Our CRISPR Guide Pages Find Validated gRNAs ...

  10. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...screens (1,2). The human and mouse GeCKO libraries, targeting over 19,000 and 20,000 human and mouse genes...GeCKO v2 pooled libraries targeting both mouse and human cells are avaliable for distribution. To learn more..."Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166 (2014): 84-87. PubMed PMID...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...

  11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...thinking about logic and analyzing human language and observed that we humans find it useful to describe and...that tends to be true about most successful human-to-human languages. Who owns computer-programming languages...? Endy: When we start thinking about languages, humans have two types of languages: one to communicate...long term, if it's going to communicate between humans (i.e., our dreams and intentions) and the genetics...storing data and computing inside living cells. He's also a co-founder of the BioBricks Foundation, an organization...elements. Open science with BioBricks The BioBrick Foundation's Linda Kahl explains the Addgene collaboration...amongst the academic community. On the BioBricks Foundation site, we have a collection of promises to not...

  12. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...-mAbs is that it can easily be engineered. Like humans, mice express multiple subclasses of IgG antibody... community.    Validation For most cloning projects, the next step would be to validate your new construct...we take a closer look at the construction and validation of some of these tools created by the Trimmer...antibodies (mAbs) that had already been thoroughly validated. The creativity in employing a variety of cloning.... 1: (A) Schematic of cloning, expression and validation pipeline. (B) Schematic shows the separate elements...Instead, the Trimmer Lab moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC...some constructs more than 90% of the colony PCR validated clones unexpectedly failed to produce functional...

  13. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now provides an opportunity to develop...for retention and replication of the vectors in human cells [8]. The EBNA-1 protein recognizes OriP and...pCXWB-EBNA-1 lacks oriP and is unable to replicate in human cells, thus expressing additional EBNA-1 transiently...PMC5118044. 2. Kim, Y., et al., The Generation of Human Induced Pluripotent Stem Cells from Blood Cells:...PMCID: PMC4989082. 3. Chou, B.K., et al., Efficient human iPS cell derivation by a non-integrating plasmid...induced pluripotent stem cells from a small amount of human peripheral blood using a combination of activated...efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and...

  14. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy Methods, 23(1), Article 1. https://...Stability and Inactivation of AAV Serotype 1 Vectors. Human Gene Therapy Methods, 28(1), Article 1. https://...Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14. https://...viral vector genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://...adeno-associated virus type 2 Reference Standard Material. Human Gene Therapy, 21(10), Article 10. https://doi.org...medium during adeno-associated virus manufacturing. Human Gene Therapy, 21(10), Article 10. https://doi.org... sufficient to detect single infectious events. Human Gene Therapy, 15(7), Article 7. https://doi.org/...

  15. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... protein-serine/threonine kinases implicated in human diseases. His spare time is spent trying to raise...access to data is important, what data should be included? We should recognize that storing data in a sharable...Discussions About Data Sharing: PLOS Data Sharing Policy Discussion PLOS’ New Data Policy: Public Access...question of how much data was necessary and reasonable. PLOS has since clarified their data sharing policy ... say that the initial data-generating lab has efficiently trawled their own data? A distinct (perhaps ...could be argued that data generators may, in light of the policy, hold onto data longer before publishing...The real cost of making data accessible A more reasonable concern with the data policy was raised by researchers...

  16. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ... by the National Institute for Child Health and Human Development (NICHD) in 1986, to enable basic research...monoclonal antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha...single sample; 3) producing recombinant, partially humanized rabbit antibodies that exhibit increased avidity...Technologies in Addgene's First 10 Years Data Freedom: The Expansion of Data Sharing in Research Publications ...

  17. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...testing. It outperformed other tRNA candidates (either from human or plant sources) in both the strength ...effective DAP array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader...efficiencies of multiplex editing at each site. A 75-nt human cysteine tRNA stood out from their initial testing...MBE with over 50% averaging editing efficiency in human cells. MBE with DAP array also exhibited reduced...type 2 diabetes, across four different types of human cells, including T cells and hematopoietic stem ...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... al., 1983), yet rarely cause severe disease in humans (and no vaccines are available on the commercial...with a linear genome between 26–45 kb in length. Human adenovirus type 5 (hAdV-C5) can be considered the...the prototype, although many viruses infecting humans (from seven species, designated as letters A–G) ...gets its name because it was first isolated from human adenoid tissue and rapidly caused the death of adenoid...Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930–6936....1953). Isolation of a Cytopathogenic Agent from Human Adenoids Undergoing Spontaneous Degeneration in ... Heinrich, W. (1983). Worldwide epidemiology of human adenovirus infections. Am J Epidemiol, 117(4), 455...

  19. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...trials in 2021. Figure 1: Schematic of the human MECP2 locus and MECP2 protein, showing mutations...mutations corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing...R270X mutation into the MECP2 gene in wild-type human iPSCs and then to successfully repair the mutation...across multiple cell types using a mouse model for a human neurological disease (Sinnamon et al., 2020). They...MECP2 guide RNA and a hyperactive version of the human RNA base editing enzyme (“Editase”), ADAR2, into...Precise CRISPR/Cas9-Mediated MECP2 Modifications in Human-Induced Pluripotent Stem Cells. Front Genet 10: ...research and discovery, Addgene and RSRT have consolidated and centralized a collection of molecular biology...

  20. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...mouse genome using CRISPR/Cas9. Similar to the human genome, the mouse genome is made up of 3 x 109 nucleotides...gene, or replacement of the murine sequence with a human gene. Advantages of using CRISPR for mouse genome...editing, such as replacement of a mouse gene with its human ortholog (greater than 5 kb), it remains to be seen...CRISPR tools were recently used to introduce a 25 kb human gene into the mouse genome. However, with only 3.4%...to develop therapeutic applications that better human health.     References Cong L, Ran FA, Cox D, Lin... Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002/0471142905...Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst...
Showing: 21 - 40 of 256 results