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...re more interested in selling a product than communicating your ideas with people or discussing science...I do a lot of public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn...own expertise and your resources for science communication opportunities like an AMA. One of the best things...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect... science communication...

...protein purification. For a more thorough introduction to pCri, please see the original publication cited...expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology... Protein purification can be one of the most stressful lab activities. Working with proteins requires...interest can save a lot of time later. Protein purification specialists recommend doing some research, notably...Gomis-Rüth, aims to take the guesswork out of protein purification through simple and efficient multiplexing. pCri...sites should one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a ...4a and -9a. 6xHis tags enable affinity purification using a Ni-NTA resin. MBP improves protein solubility...

... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...chief goals. First, SITN works to bridge the communication gap between scientists and the public and help... SITN helps graduate students develop their communication skills and engage with the public. The organization...Bio-Medley) to showcase SITN’s strength in the biomedical sciences and decided to offer a varied format...students, these opportunities to improve our communication and organizational skills provide great training... science communication...

... the types of viruses used as biological tools.   Viruses as biological tools In nature, viruses infect...enough to ensure the virus can replicate and spread. Instead, viruses replicate by hijacking the host’s system...them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...Capacity (~4.5kb From basic science to clinical applications, viruses are true bench to bedside tools... why viruses are such wonderful scientific and medical tools. As scientists, we can manipulate what this...effectively infect a wide range of cell types that are typically ‘difficult to transfect’, such as primary cells...straightforward across applications, while electroporation of a human, for example, isn’t a reasonable option. Since...

...ideas about the controversial medical issues. Participants gave significantly more favourable ratings to...Further, when science is communicated in a format that is accessible, that communication often neglects the ...SciCommSummer Scicomm Series: Modes of CommunicationCareers in Science Communication: Science Writing... past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers. In high... to save a few days’ worth of capsules to self-medicate with when they get a cold. A primary school teacher...boasted to me that he never eats “anything with chemicals in it,” leaving me to wonder how he has survived...dihydrogen monoxide (H2O). Studying science communication as a PhD student Fortunately, by the time I ...

...run in triplicate.  The nice thing about qPCR is that since it measures relative quantification - that... not need to be run in triplicates. ddPCR is an extremely accurate application, even in areas of the genome...Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview and Applications Resources on Addgene.org...blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative/real-time... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ... on and so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either...range of RQ values as noted above.   In both applications, results can be affected by DNA quality and ...

...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but....” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016, pp. 1059–69. Crossref...Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref...Libraries.” ACS Synthetic Biology, vol. 6, no. 6, American Chemical Society (ACS), 15 Mar. 2017, pp. 1016–25. ...Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015, pp. 975–86. Crossref...” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp. 3393–405. ....” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, pp. 839–43. Crossref...

...to a) figure out the sources of miscommunication and b) find practical solutions.  My PhD prepared me... for this task. Good scientists learn how to communicate their work differently based on their audience... family is going to vary widely. The way you communicate your science at a poster is going to vary depending...two main problems with Addgene’s cross team communication. Although these real world examples did not ... for the container to be used in cross-team communication A list of “AKA” names, or jargon terms, for ...types or purposes of each given container, when applicable Any other relevant information Prescribing an...tracking system. Though you might not have to communicate with a team of software engineers in your lab...

...background, which means that each mouse is genetically identical and all mice of the strain are homozygous...create a limited but genetically diverse gene pool. Outbred strains are strategically bred to maintain maximum...using the same nuclease may not result in genetically identical mice. Thus, scientists must genotype each...organism used to understand mammalian traits and genetically engineered mouse models provide researchers with...useful and adaptable tools to perform basic and preclinical research. For scientists new to using mouse models...in research can fall under one of two broad classifications: inbred or outbred. Inbred strains share a ...) National Institutes of Health (NIH) Swiss. Genetically engineered mice Mice can be engineered by inserting...

... Predoctoral Fellow at University of Rochester Medical Center. During development, complex genetic programs...genetic underpinnings of these processes has been complicated by the lack of precise genetic tools for modulating...central nervous system (CNS). To address this technological gap, a trio of recent papers describe the development...recent advancements and offer commentary on their application to neuroscience research. What is CRISPR-based...with different untranslated or coding regions. Typically, each gene isoform has a distinct transcriptional...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly...necessitates such molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used ...

...some tips for performing this purification.  Check out the AAV Purification protocol page for more detailed...! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation This...This video goes over AAV vector purification using an iodixanol column gradient. We'll show the loading... tissue culture as it is commonly called, is a critical tool for many scientists. Mammalian cells provide...provide scientists with the means to study biological processes on the cellular level instead of having ...

... the Communicating Science Conference series for graduate students. I believe that communication is the...skill in science communication over time.  Like most skills, great science communication comes with practice...able to communicate with their colleagues and peers. Scientists also need to be able to communicate to expert...careers in science communication The strength of story telling Science communication: 9 strategies to get... world is the value that you can succeed in communicating. Even the most rigorous, insightful, and novel...scientist's career is mediated by some kind of communication to another person.  In an interview setting,...you do to ensure that your skills in science communication will be a positive asset throughout your career...

...repair Adapter Ligation Clean up/Size selection Amplification Clean up  I found this kit easy to use because...DNA we started the process with, we got great amplification of our sample libraries. NEB also gave us a ...mostly used to make new libraries as opposed to replicating them like we do at Addgene), going to the workshop...

...Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs) have become a vital tool...potential (AP) spiking events are indicated below the trace; asterisk indicates a single AP. B) Averaged fluorescence...just the stuff that makes bones strong; it plays critical roles in nerve and muscle function, hormone release...toolbox, our team recently developed a new calcium indicator, OCaMP. The challenge: Expanding the fluorescent... signals in the brain. For this to work, each indicator needs to emit a different color of light and be...FPbase.org. Scientists have developed calcium indicators spanning nearly the entire visible spectrum (...gold standard for green fluorescence, while red indicators jRGECO1a and jRCaMP1a are widely used as well...

...other scientists to use? We take this Hold for Publication policy seriously and appreciate the trust you...of July 1, 2025, we are updating our Hold for Publication policy with two changes that will help us ensure...hold if they are clearly described in a journal publication. Plasmids will be released 3 years after the ...keep things moving internally. And remember, a publication isn’t required to share your plasmids! You can...can make your deposit available before publication and we can update the plasmids’ citation when the paper...

...reproducibility crisis.” Results in preclinical, biomedical and psychological sciences were called into question...researchers.There is both theoretical and empirical evidence (in psychology, cancer biology, pre-clinical life science...after credible attempts to replicate major findings could not be replicated by other researchers.There...published research is difficult to replicate.  Why is replicating previous research findings so difficult...can be difficult to replicate. Unclear methods Until someone attempts to replicate the results of another...practices (QRPs) can quickly lead us to find statistically significant results that are not robust. Sampling ...an attempt to present only the most novel or significant findings, and combined with powerful cognitive...

...placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically control the depolarization...researchers to label just a fraction of a single genetically-defined subset of cells. By labeling fewer cells...While transgenic animals are commonly used to specifically manipulate a cell type of interest in an organism...Drosophila require heat-shocking the system or using chemical inducers of gene expression (del Valle Rodríguez...cells are not labeled. PhiC31, a recombinase. It specifically integrates an attP site into an attB site. In...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image ...Image from Isaacman-Beck et al., 2019. Applications for SPARC SPARC technology is versatile and can be ...

...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...Here, we will explain how practical brightness is determined and why practical brightness is a better criterion...criterion than theoretical brightness for selecting the brightest fluorescent protein. Theoretical brightness...higher the number, the higher the theoretical brightness. The theoretical brightness often appears in tables...proteins based on their practical brightness in your experimental set up. The practical brightness takes all...same reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...which is a key determinant of the practical brightness. Therefore, practical brightness provides a better ...

...science communication are as varied as the field itself. Among other things, science communication comprises...suspect that science communication might be for you, don’t wait. Start communicating now. These 9 strategies...museums, zoos or botanical gardens. You can also work or volunteer for a science communication organisation...project. Science communication opens doors Getting involved in science communication has been the most...career researcher looking to break into science communication? Everybody goes about this in their own way....hashtag #scicomm and start following science communication societies like @BritSciAssoc, @AAAS, @Stempra.... Use Twitter to promote your other science communication activities, e.g. tweet it when you publish a...

...about Julie Cicalese  Ms. Cicalese served as the Chief Human Resources Officer for Harvard Medical School ...excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief Human...Human Resource Officer for Harvard Medical School. The addition of Cicalese to the Board of Directors brings...Human Resources Association, Association of American Medical Colleges and the Society for Human Resource...When I was working in Human Resources at Harvard Medical School, Joanna was my colleague. She mentioned ... science but my prior HR experience at Harvard Medical School means I understand the needs of scientists...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...