...system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression of dCas9...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...

... are named for soup ingredients! From pSOUP to mCherry, satisfying ingredients reagents permeate the molecular...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...

...cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

...channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The resulting...membrane, a threshold is reached and the neuron fires, sending the electrical signal down the axon (Lodish...