... like C. difficile. In 2014, Citorik et al. and Bikard et al. developed sequence-specific, antimicrobial...phagemids were cytotoxic only for resistant bacteria. Bikard et al. used a phagemid system to target virulent... the phagemid or received a defective phagemid; Bikard et al. did not observe any mutations at the target... Find plasmids from this paper at Addgene. 2. Bikard, David, et al. “Exploiting CRISPR-Cas nucleases...

... (RMLC) of nonmuscle myosin II (Karess et al., 1991, Jordan and Karess, 1997), which contains the regulatory...Central PMCID: PMC2395242. Jordan, Pascale, and Roger Karess. "Myosin light chain–activating phosphorylation...Development 130.3 (2003): 563-573. PubMed PMID:12490562. Karess, Roger E., et al. "The regulatory light chain of...

...genome-wide inhibition library from David Bikard’s lab. The Bikard library contains ~93,000 unique gRNAs ...Read the paper in Nature Communications Find the Bikard E. coli Genome-wide Inhibition Library CRISPR ...

...Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth... serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype pAAV_hSyn-PdCO-mScarlet-WPRE...

...expression systems have been improved for both eukaryotic and prokaryotic expression in recent years, with many...cell-free expression of proteins in both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He...sequence bypasses the 5′ mRNA cap required for eukaryotic systems and instead directly engages the ribosome...efficiently blocks read-through transcription in eukaryotic cells. pXPG contains the Luc+ luciferase gene...

...learn more! Background: Argonaute and NgAgo In eukaryotes, Argonaute proteins play an important role in... RNA. Argonaute proteins are also present in prokaryotes, and some of these proteins protect against invading...., et al.  “DNA-guided DNA interference by a prokaryotic Argonaute.” Nature. 507(7491) (2014):258-61. ...

...single RFP-labeled osk and Oskar protein were detected at the posterior pole. Oskar protein levels were negatively...examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK reporter was...

...AAVrg pAAV-Ef1a-DIO EYFP Karl Deisseroth 11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2...pAAV-hSyn-DIO-EGFP Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell...

...occurring restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The...degraded by restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction...Impact on cloning and digests Although not all prokaryotic DNA is methylated to the same level, the potential...

...collaborations. Summarized by Professor Michael Karin from UCSD: “The problem with cancer research is ...studies! This was followed by a talk from Professor Karin about alcohol and obesity as driving factors for.... Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in cancer prevention and...

...efficient tRNA processing is common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. ... or pre-tRNAs are cleaved by RNase P and Z in eukaryotes (E in bacteria), and only a few elements of tRNAs...

...Many thanks to our guest blogger Oskar Laur from Emory University. Oskar Laur, PhD runs Emory University...This post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo...

...whether and how organisms respond to magnetic fields (Karki et al., 2021; Kattnig et al., 2016), and some previous... cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka et al.,... andrewgyork.github.io/gfp_magnetofluorescence. Karki, N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes...

...//doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment of...Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F (2019) RNA-guided DNA insertion...doi.org/10.1126/science.aax9181 Strecker J, Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment...

...their initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and.... Using computational observations, Koonin and Makarova found that Cas7-11 appeared to have derived from...Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable...

...helps scientists achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of...tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017)  Table 1 (Gutierrez-Guerrero et ...Viruses 12:1016 . https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors...

... at Addgene. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

... S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev...10.1261/rna.054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

...codon optimize genes from eukaryotic organisms for expression in prokaryotic systems or yeast (Lanza et...base-pairing mechanism for translation initiation in eukaryotes. Nat Struct Mol Biol 13:30–34 . https://doi.org...

...Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara et al., 2013 Skeletal muscle MyoAAV Tabebordbar...1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne, L. C., Klimczak, R. R., Visel, M., ...Hantman, A. W., Looger, L. L., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient...