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We narrowed to 24 results for: lentiviral envelope plasmid

Showing: 21 - 24 of 24 results

  1. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...transfer plasmid), the envelope (Env) gene on a separate plasmid (envelope plasmid), and the polymerase ...different plasmids. For a retroviral system this looks like the genetic cargo in one plasmid (the transfer...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection... transfection of all three plasmids into a producer cell line, such as HEK-293T, production of viral particles... and Pol genes are not encoded in the transfer plasmid, the component that physically integrates into ... retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific safety...services Addgene Biosafety Guide Addgene Viral Plasmids Which Type of Virus Should I Use? Resources...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses, so they are resistant to alcohol-based...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...HSV can). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...
Showing: 21 - 24 of 24 results